Oligoclonal T cell expansion in myelodysplastic syndrome: evidence for an autoimmune process
Oligoclonal T cell expansion in myelodysplastic syndrome: evidence for an autoimmune process
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DOI：
10.1016/s0145-2126(01)00083-2
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发表时间：
2001-12-01
影响因子：
2.7
通讯作者：
Barrett, AJ
中科院分区：
文献类型：
--
作者：
Epperson, DE;Nakamura, R;Barrett, AJ
越来越多的证据表明骨髓增生异常综合征（MDS）的骨髓衰竭是免疫介导的。我们研究了 MDS 患者，以寻找寡克隆或克隆。 T 细胞的扩增表明自身免疫过程。我们使用基于 PCR 的技术（光谱分型）来表征 MDS 中的 T 细胞库（n = 15；9 例 RA、4 例 RARS、2 例 RAEB），并将结果与年龄匹配的健康捐献者 (n = 20) 和患有血红蛋白病的输血依赖 (TD) 患者 (n = 5) 进行比较。我们发现，与对照组相比，MDS 患者的 V β 谱偏态数量明显增多。在外周血 T 细胞中，MDS 患者检查的 60/345 V β 谱与 TD 对照中的 3/115 V β 谱相比存在偏差（P < 0.0001），而年龄匹配对照中则为 58/460 V β 谱（P = 0.05）。对偏斜 V β 谱内 J β 区域的研究表明，MDS 中优先使用 J β 2.1，而对照中则在整个 J β 谱上广泛分布，这与非随机 T 细胞克隆一致。 MDS 的扩展。这些发现进一步证明 T 细胞介导的免疫过程是 MDS 的一个特征。由爱思唯尔科学有限公司出版
There is accumulating evidence that the marrow-failure of myelodysplastic syndrome (MDS) is immune-mediated. We studied patients with MDS to look for oligoclonal or clonal. expansion in T cells indicative of an autoimmune process. We used a PCR-based technique (spectratyping) to characterize the T cell repertoire in MDS (n = 15; 9 RA, 4 RARS, 2 RAEB) and compared results with age-matched healthy donors (n = 20) and transfusion-dependent (TD) patients with hemoglobinopathy (n = 5). We found a significantly higher number of skewed V beta profiles in the MDS patients compared with controls. In peripheral blood T cells, 60/345 V beta profiles examined were skewed in MDS patients compared with 3/115 V beta profiles in TD controls (P < 0.0001), and 58/460 V beta profiles in age-matched controls (P = 0.05). A study of J beta region within the skewed V beta profiles revealed preferential usage of J beta 2.1 in MDS in contrast with a wide distribution over the entire J beta spectrum in controls, consistent with non-random T cell clonal. expansion in MDS. These findings provide further evidence that T cell mediated immune processes are a feature of MDS. Published by Elsevier Science Ltd.