Phenotypic and Functional Study of Human Plasmacytoid Dendritic Cells.
Phenotypic and Functional Study of Human Plasmacytoid Dendritic Cells.
复制标题
DOI：
10.1002/cpz1.50
复制
发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Su L
中科院分区：
文献类型：
--
作者：
Li G;Cheng L;Su L
浆细胞样树突状细胞（pDC）代表骨髓来源的细胞的独特谱系，其主要以稳态存在于血液和淋巴器官中，但也存在于感染、炎症和癌症的部位。本单元中的基本方案描述了1）检测和定量外周血中的人pDC; 2）通过磁激活细胞（MACS）分选和荧光激活细胞（FACS）分选分离人pDC; 3）通过用TLR 7/9激动剂刺激来评价人pDC功能; 4）通过流式细胞术检测人源化小鼠的淋巴组织中的人pDC; 5）人pDC在hu小鼠体内的功能研究;和6）使用靶向人pDC的单克隆抗体在hu小鼠体内特异性消耗人pDC。因此，这些检测方法，提供了全面的方法，在体外的表型和功能的研究，并在人浆细胞样树突状细胞在hu-小鼠体内的调查。
Plasmacytoid dendritic cell (pDC) represents a distinct lineage of bone-marrow–derived cells that reside mainly in blood and lymphoid organs in the steady state but are also present in sites of infection, inflammation, and cancer. The Basic Protocol in this unit describes 1) detection and quantitation of human pDC in peripheral blood; 2) isolation of human pDC by magnetic-activated cell (MACS) sorting and fluorescent-activated cell (FACS) sorting; 3) evaluation of human pDC function by stimulation with TLR7/9 agonists; 4) detection of human pDC in lymphoid tissues of humanized mice by flow cytometry; 5) functional study of human pDC in hu-mice in vivo; and 6) specific depletion of human pDC in vivo in hu-mice using monoclonal antibody targeting human pDC. These assays, therefore, provide comprehensive methods to the phenotypic and functional study in vitro, and the investigation of human plasmacytoid dendritic cells in hu-mice in vivo.