Effects of Bone Marrow Mesenchymal Stem Cells-Conditioned Medium on Tibial Partial Osteotomy Model of Fracture Healing in Hypothyroidism Rats.
Effects of Bone Marrow Mesenchymal Stem Cells-Conditioned Medium on Tibial Partial Osteotomy Model of Fracture Healing in Hypothyroidism Rats.
复制标题
骨髓间充质干细胞调节培养基对甲状腺功能减退症大鼠骨折愈合的胫骨部分截骨模型的影响。
DOI：
10.22034/ibj.22.2.90
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发表时间：
2018-03
影响因子：
--
通讯作者：
Fadaei Fathabady F
中科院分区：
文献类型：
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作者：
Sefati N;Norouzian M;Abbaszadeh HA;Abdollahifar MA;Amini A;Bagheri M;Aryan A;Fadaei Fathabady F
甲状腺功能减退与骨转换功能障碍、成骨细胞骨形成和破骨细胞骨吸收减少有关。间充质干细胞（MSCs）分泌多种刺激骨再生的因子和细胞因子。本研究旨在探讨骨髓间充质干细胞条件培养基（MSCs-conditioned medium， CM）对雄性甲状腺功能减退大鼠骨缺损的影响。将24只雄性大鼠随机分为(1)甲状腺功能减退+骨缺损（HYPO）组、(2)甲状腺功能减退+骨缺损+ CM （HYPO + CM）组和(3)无甲状腺功能减退+骨缺损（对照）组。术后4周，切除右胫骨，立即进行生物力学和组织学检查。结果显示，甲状腺功能减退大鼠截骨部位的弯曲刚度（32.64±3.99）、最大受力（14.63±1.89）、高应力负荷（7.59±2.31）、能量吸收（12.68±2.12）较正常对照组和甲状腺功能减退+条件中等组显著降低（p < 0.05）。甲状腺功能减退大鼠截骨部位骨小梁体积（3.86±3.88）和骨细胞数量（5800±859.8）也显著低于对照组和甲状腺功能减退+条件中组（p < 0.01和p < 0.02）。本研究提示，CM可促进甲状腺功能减退大鼠骨折再生，加速截骨部位骨愈合。
Hypothyroidism is associated with dysfunction of the bone turnover with reduced osteoblastic bone formation and osteoclastic bone resorption. Mesenchymal stem cells (MSCs) secrete various factors and cytokines that may stimulate bone regeneration. The aim of this study was to determine the effects of MSCs-conditioned medium (CM) in hypothyroidism male rats after inducing bone defect. In this study, 24 male rats were randomly assigned to three groups: (I) hypothyroidism + bone defect (HYPO), (II) hypothyroidism + bone defect + CM (HYPO + CM), and (III) no hypothyroidism + bone defect (control). Four weeks after surgery, the right tibia was removed, and immediately, biomechanical and histological examinations were performed. The results showed a significant reduction in bending stiffness (32.64 ± 3.99), maximum force (14.63 ± 1.89), high stress load (7.59 ± 2.31), and energy absorption (12.68 ± 2.12) at the osteotomy site in hypothyroidism rats in comparison to the control and hypothyroidism + condition medium groups (p < 0.05). There was also a significant decrease in the trabecular bone volume (3.86 ± 3.88) and the number of osteocytes (5800 ± 859.8) at the osteotomy site in hypothyroidism rats compared to the control and hypothyroidism + condition medium groups (p < 0.01 and p < 0.02, respectively). The present study suggests that the use of the CM can improve the fracture regeneration and accelerates bone healing at the osteotomy site in hypothyroidism rats.