Genome‐wide expression quantitative trait loci studies facilitate isolation of causal genes controlling panicle structure
Genome‐wide expression quantitative trait loci studies facilitate isolation of causal genes controlling panicle structure
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全基因组表达数量性状位点研究有助于分离控制穗结构的因果基因
DOI：
10.1111/tpj.14726
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Matsuoka Makoto
中科院分区：
文献类型：
--
作者：
Wang Fanmiao;Yano Kenji;Nagamatsu Shiro;Inari‐Ikeda Mayuko;Koketsu Eriko;Hirano Ko;Aya Koichiro;Matsuoka Makoto
水稻（Oryza sativa L.）穗是决定产量的重要因素，阐明穗结构的遗传控制对于满足育种计划中高产的要求是非常重要的。利用82个来自越光和羽崎的回交自交系进行了数量性状基因座（QTL）研究，共检测到25个穗部形态性状的68个QTL。获得了BILs花序分生组织的基因表达谱。表型QTL（pQTL）和表达QTL（eQTL）的组合分析揭示了pQTL和eQTL之间的共定位，与表型性状和基因表达水平之间的显著相关性一致。结合pQTL和eQTL数据，确定了2个控制穗部结构的基因：OsMADS 18调节一次穗轴的平均长度，OsFTL 1对二次穗总粒数、每穗粒数、株高和剑叶长度具有多效性效应。在RNA干扰敲低的植物和过表达株系中证实了表型。将pQTL和eQTL结合起来分析，有助于鉴定与水稻成穗相关的基因。
The morphology of rice (Oryza sativaL.) panicles is an important determinant of grain yield, and elucidation of the genetic control of panicle structure is very important for fulfilling the demand for high yield in breeding programs. In a quantitative trait locus (QTL) study using 82 backcross inbred lines (BILs) derived from Koshihikari and Habataki, 68 QTLs for 25 panicle morphological traits were identified. Gene expression profiling from inflorescence meristems of BILs was obtained. A combination of phenotypic QTL (pQTL) and expression QTL (eQTL) analysis revealed co‐localization between pQTLs and eQTLs, consistent with significant correlations between phenotypic traits and gene expression levels. By combining pQTL and eQTL data, two genes were identified as controlling panicle structure:OsMADS18modulates the average length of the primary rachis andOsFTL1has pleiotropic effects on the total number of secondary rachides, number of grains per panicle, plant height and the length of flag leaves. Phenotypes were confirmed in RNA interference knocked‐down plants and overexpressor lines. The combination of pQTL and eQTL analysis could facilitate identification of genes involved in rice panicle formation.