A cytoplasmic protein inhibits the GTPase activity of H-Ras in a phospholipid-dependent manner.
A cytoplasmic protein inhibits the GTPase activity of H-Ras in a phospholipid-dependent manner.
复制标题
细胞质蛋白以磷脂依赖性方式抑制 H-Ras 的 GTP 酶活性。
DOI：
10.1126/science.2237442
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Stacey,DW
中科院分区：
文献类型：
--
作者：
Tsai,MH;Yu,CL;Stacey,DW
已经确定了一种细胞质蛋白，它可以抑制细菌合成的细胞H-RAS蛋白的鸟苷三磷酸酶(GTPase)活性。这种GTP酶抑制蛋白能够抵消GTP酶激活蛋白(GAP)的活性，GAP被认为是RAS活性的负调节因子。GTP酶抑制蛋白与脂质的相互作用进一步支持了其潜在的生物学意义。作为有丝分裂刺激的结果，细胞中产生的磷脂增加了GTP酶抑制蛋白的活性，也抑制了GAP的活性。因此，这些脂类与这两种调节蛋白的相互作用往往会增加RAS的生物活性，并刺激细胞增殖。
A cytoplasmic protein has been identified that inhibits the guanosine triphosphatase (GTPase) activity of bacterially synthesized, cellular H-Ras protein. This GTPase inhibiting protein is able to counteract the activity of GTPase activating protein (GAP), which has been postulated to function as a negative regulator of Ras activity. The potential biological importance of the GTPase inhibiting protein is further supported by its interaction with lipids. Phospholipids produced in cells as a consequence of mitogenic stimulation increase the activity of the GTPase inhibiting protein, as well as inhibit the activity of GAP. The interaction of such lipids with each of these two regulatory proteins would, therefore, tend to increase the biological activity of Ras and stimulate cell proliferation.