Fecal hydrogen sulfide production in ulcerative colitis
Fecal hydrogen sulfide production in ulcerative colitis
复制标题
DOI：
10.1111/j.1572-0241.1998.083_c.x
复制
发表时间：
1998-01-01
影响因子：
9.8
通讯作者：
Levitt, MD
中科院分区：
文献类型：
--
作者：
Levine, J;Ellis, CJ;Levitt, MD
硫化物是硫酸盐还原菌的产物，已被认为在溃疡性结肠炎中起病因作用。溃疡性结肠炎粪便中硫酸盐还原菌的数量和活性增加，但硫化物仅适度增加。然而，粪便硫化物主要以挥发性的、高毒性的H2S形式存在，其从粪便迅速移动到周围的气体。我们的目的是量化粪便释放的H2S和其他挥发性物质（CO2，H-2，CH 4，甲硫醇和二甲硫醚）。研究方法：将来自25名溃疡性结肠炎受试者和17名对照者的粪便样本在4 L容器中孵育，并在24 h内间隔评估气体释放。结果：溃疡性结肠炎粪便的H2S释放在每个测量点与正常粪便相比升高3-4倍（在24 h时p < 0.003）。唯一的其他显著差异是溃疡性结肠炎粪便在1 h时的CO2释放增加。补充粪便匀浆含硫底物表明，有机化合物（粘蛋白，半胱氨酸，牛磺胆酸盐）提供更容易利用的底物H2S生产比硫酸盐。结论：增加的H2S释放是溃疡性结肠炎粪便的相对局部化的代谢异常，这种增加的H2S可能反映粪便细菌和/或底物可用性的异常。(C)1998年，我Cell.胃肠病学
Sulfide, a product of sulfate-reducing bacteria, has been proposed to play an etiologic role in ulcerative colitis, Ulcerative colitis feces have increased numbers and activity of sulfate-reducing bacteria, but only modestly increased sulfide. However, fecal sulfide exists largely in the volatile, highly toxic H2S form that moves rapidly from feces to surrounding gas. Our aim was to quantify the fecal release of H2S and other volatiles (CO2, H-2, CH4, methanethiol, and dimethylsulfide). Methods: Fecal samples from 25 subjects with ulcerative colitis and 17 controls were incubated in 4-L containers, and gas release was assessed at intervals over 24 h. Results: H2S release by ulcerative colitis feces was elevated 3-4-fold at every measurement point compared with normal feces (p < 0.003 at 24 h). The only other significant difference was increased CO2 release by ulcerative colitis feces at 1 h. Supplementation of fecal homogenates with sulfur-containing substrates showed that organic compounds (mucin, cysteine, taurocholate) provided more readily utilizable substrate for H2S production than did sulfate. Conclusions: increased H2S release is a relatively localized metabolic aberration of ulcerative colitis feces, This increased H2S may reflect abnormalities of the fecal bacteria and/or substrate availability. (C) 1998 by Am. Cell. of Gastroenterology.