Confirmation of HCV viremia using HCV RNA and core antigen testing on dried blood spot in HIV infected peoples who inject drugs in Vietnam
Confirmation of HCV viremia using HCV RNA and core antigen testing on dried blood spot in HIV infected peoples who inject drugs in Vietnam
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DOI：
10.1186/s12879-018-3529-3
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发表时间：
2018-12-04
影响因子：
3.7
通讯作者：
Tuaillon, Edouard
中科院分区：
文献类型：
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作者：
Truong Tam Nguyen;Lemee, Veronique;Tuaillon, Edouard
背景干血斑（DBS）上采集的血液样本进行核酸检测和HCV核心抗原（HCVcAg）检测是两种可能有助于低收入和中等收入国家获得HCV诊断的方法。在这项研究中，我们评估了HCV RNA和HCV抗原检测DBS在HIV/HCV共感染的人谁注射药物在Vietnam.MethodOne和四个HIV/HCV血清阳性患者在门诊治疗管理在海防越铁医院被列入本研究从2014年2月至3月结果86例受试者血清HCV RNA检测阳性，中位数（IQR）：6.9 log（10）IU/ml（5.6-7.4 log（10）IU/ml）。基因型包括57 G1（69%）、3G 3（4%）和22 G6（27%）。在86例慢性丙型肝炎受试者中，79例DBS标本中检出HCV RNA（灵敏度92.5%; 95%CI：85.1-96.9%）。DBS和血清上的HCV RNA水平中度相关（r=0.24; p=0.05），表明运输和储存条件导致HCV RNA降解。HCVcAg在75/86 dB标本中检出（敏感性：87.2%，95%CI：78.3-93.4%），与血清HCVRNA水平呈强正相关（r=0.80; P
BackgroundNucleic acid tests performed on blood samples collected on Dried Blood Spot (DBS) and detection of HCV core antigen (HCVcAg) are two approaches that may facilitate access to HCV diagnosis in low and middle incomes countries. In this study we evaluate HCV RNA and HCV antigen testing on DBS in HIV/HCV co-infected peoples who inject drugs in Vietnam.MethodOne hundred and four HIV/HCV seropositive patients managed in outpatient care at the Haiphong Viet Tiep hospital were included in this study from February to March, 2014 (ANRS 12262 study).ResultsEighty-six subjects were tested positive for HCV RNA in serum, median (IQR): 6.9 log(10) IU/ml (5.6-7.4 log(10) IU/ml). Genotypes consisted of 57G1 (69%), 3G3 (4%), and 22G6 (27%). HCV RNA was detected on DBS specimens in 79 out 86 subjects with chronic hepatitis C (sensitivity 92.5%; 95% CI: 85.1-96.9%). HCV RNA level on DBS and serum was moderately correlated (r=0.24; p=0.05) suggesting a degradation of HCV RNA due to transportation and storage conditions. HCVcAg was detected in 75/86dB specimens (sensitivity: 87.2%; 95% CI: 78.3-93.4%), with a strong positive relationship between DBS HCVcAg and serum HCV RNA levels (r=0.80; P