CHARACTERIZATION OF A NEW FILAMENTOUS PHAGE CF FROM XANTHOMONAS-CITRI
CHARACTERIZATION OF A NEW FILAMENTOUS PHAGE CF FROM XANTHOMONAS-CITRI
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DOI：
10.1099/0022-1317-46-2-277
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发表时间：
1980-01-01
影响因子：
3.8
通讯作者：
KUO, TT
中科院分区：
文献类型：
--
作者：
DAI, H;CHIANG, KS;KUO, TT
从X.柑橘。该噬菌体命名为Cf，测量值为10075 ± 10075。985次10-1 12 h内在宿主上形成一个异常清晰的微小噬菌斑。[感染后]。感染的CF既不杀死也不彻底阻止宿主细胞的繁殖。宿主细胞分裂的速度严重减慢。该噬菌体表现出非常窄的宿主范围，并且在常规用于制备其它丝状噬菌体的常规Tris缓冲液和合成培养基中不稳定。根据核苷酸组成分析（也显示Cf含有不寻常的核苷酸）、热变性特征和羟基磷灰石柱洗脱模式，Cf的基因组为单链DNA。在宿主细胞中的噬菌体增殖过程中，检测到单链病毒DNA、复制型[RF] I和RFII。丙烯酰胺凝胶电泳结果表明，病毒、RFI和RFII DNA种类的大小和构象与另一种丝状噬菌体Xf的对应物相同。
A new filamentous phage was isolated from X. citri. This phage, designated Cf, measures 10075 .+-. 985 .times. 10-1 nm in length and forms an unusually clear by tiny plaque on the host within 12 h p.i. [post-infection]. Infection by Cf neither killed nor drastically prevented host cells from propagation. The rate of host cell division was severely retarded. This phage exhibited an exceedingly narrow host range and was unstable in conventional Tris buffer and synthetic medium routinely used for the preparation of other filamentous phages. Based on nucleotide composition analysis (which also revealed that Cf contains an unusual nucleotide), thermal denaturation characteristics and the hydroxyapatite column elution pattern, the genome of Cf was single-stranded DNA. During phage multiplication in host cells single-stranded virus DNA, replicative form [RF] I and RFII were detected. As shown by acrylamide gel electrophoresis, the size and conformation of the virus, RFI and RFII DNA species were the same as their counterparts of another filamentous phage Xf.