Nodulation and nitrogen fixation by fast- and slow-growing rhizobia strains of soybean on several temperate and tropical legumes
Nodulation and nitrogen fixation by fast- and slow-growing rhizobia strains of soybean on several temperate and tropical legumes
复制标题
快速和缓慢生长的大豆根瘤菌菌株在几种温带和热带豆科植物上的结瘤和固氮作用
作者：
M. Chamber;E. E. Iruthayathas
以大豆为试材，对3株不同来源的慢生型大豆根瘤菌（G3、USDA-110和KUL-150）和2株快生型大豆根瘤菌（USDA-192和USDA-193）进行了田间试验。梅里尔）、豇豆（Vigna unguiculata）、绿豆（Phaseolus radiata）、四棱豆（Psophocarpus tetragonolobus）和菜豆（Phaseolus vulgaris）。fredii菌株与B.在现代欧洲大豆栽培品种，即Fiskeby V.中，其他西方培育的大豆没有被这些R. fredii菌株。所有的大豆根瘤菌都能在豇豆和绿豆中产生根瘤。fredii菌株在豇豆中表现出有效的固氮作用，特别是USDA-193，产生的茎干重大于B.日本的它们的共生性能。fredii菌株与大豆和其他豆类的关系表明，它们应被置于缓慢生长的B.日本根瘤菌（B. japonicum）和牛肝菌根瘤菌（Cowpearhizobium sp.）的吸氢活性表明，这些基因的表达可能受到宿主的影响。日本血吸虫菌株。慢生根瘤菌的硝酸盐还原能力显著高于R. fredii在结节。
Three slow-growingBradyrhizobium japonicum (G3, USDA-110 and KUL-150) of diverse origins and two fast-growing strains ofRhizobium fredii (USDA-192 and USDA-193) were tested with a cropped soybean (Glycine max L. Merrill) cultivar, two cowpeas (Vigna unguiculata), one mung-bean (Phaseolus radiata), one winged-bean (Psophocarpus tetragonolobus) and one field bean (Phaseolus vulgaris) varieties.TheR. fredii strains nodulated and fixed Nitrogen as effectively as the strains ofB. japonicum in a modern european soybean cultivar, namely Fiskeby V. The other western bred soybeans tested were not nodulated by theseR. fredii strains. All of the soybean rhizobia produced nodules in both cowpeas and in mung-bean; theR. fredii strains showed effective N2-fixation in the cowpeas, particularly USDA-193, yielding shoot dry weights greater than those from theB. japonicum. The symbiotic performance of theR. fredii strains with soybean and other legumes indicated that they should be placed in an intermediate group between the slow-growingB. japonicum and cowpearhizobium sp.The hydrogen uptake activites suggested a possible host effect on the expression of such genes in one out of theB. japonicum strains tested. Furthermore, the slow-growing rhizobia showed significantly higher nitrate-reduction than theR. fredii in the nodules.