Enhancement of 17α-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-β-cyclodextrin complexation technique
Enhancement of 17α-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-β-cyclodextrin complexation technique
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DOI：
10.1016/j.jsbmb.2008.10.003
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
J. Manosroi;Suda Saowakhon;A. Manosroi
中科院分区：
文献类型：
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作者：
J. Manosroi;Suda Saowakhon;A. Manosroi
研究了羟丙基-β-环糊精（HPβCD）包合物结合通气和超声技术对孕酮生物转化合成17α-羟孕酮（17α-HP）的促进作用。采用共蒸发法制备了胆甾酮-羟丙基-β-环糊精包合物。在新月弯孢菌ATCC 12017中，24 h时17α-HP的产率为11.26±0.64%。复合态P经40 kHz超声处理5s和通气处理后，17α-HP的产率可达72.92±4.28%，分别是未复合态P和复合态PC的6.5倍和1.3倍。与HPβCD络合增加P的水溶性是提高17α-HP产率的主要因素，而通气对P的影响大于PC。超声处理没有显著增加P和PC的产物产率。当在PC系统中同时使用曝气和超声处理时，产物产率比P的产率显著增加。本研究的结果可应用于其他水溶性差的前体物的生物转化。
An enhancement of 17α-hydroxyprogesterone (17α-HP) production from progesterone by biotransformation using hydroxypropyl-β-cyclodextrin (HPβCD) complexation together with aeration and sonication technique was demonstrated. The progesterone–hydroxypropyl-β-cyclodextrin complex was prepared by co-evaporation method. The percentage yield of 17α-HP from P of 11.26±0.64% at 24h was observed in Curvularia lunata ATCC 12017. In the complex form of P, together with sonication at 40kHz for 5s and aeration, the yield of 17α-HP was increased to 72.92±4.28% which was about 6.5 and 1.3 times of that from the uncomplexed (P) and the complexed (PC), respectively without sonication and aeration. The increased aqueous solubility of P by complexation with HPβCD was the main factor which increased the yield of 17α-HP, while aeration had more effect on P than PC. Sonication did not significantly increased the yield of the product from both P and PC. When both aeration and sonication were used in the PC system, the product yield was increased significantly more than that from P. The result from this study can be applied for the biotransformation of other poor aqueous soluble precursors.