A proteomics study of barley powdery mildew haustoria
A proteomics study of barley powdery mildew haustoria
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DOI：
10.1002/pmic.200800645
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发表时间：
2009-06-01
期刊：
影响因子：
3.4
通讯作者：
Thordal-Christensen, Hans
中科院分区：
文献类型：
--
作者：
Godfrey, Dale;Zhang, Ziguo;Thordal-Christensen, Hans
许多具有重要经济意义的真菌和卵菌植物病原体通过吸器以寄主为食，它们将吸器放置在活的植物细胞内。其潜在机制尚不清楚，部分原因是难以制备吸器。因此，我们开发了一种从大麦白粉病真菌（Blumeria graminis f.sp）中分离吸虫的方法。hordei使用Bgh)。我们随后旨在通过对吸器蛋白质组的研究来了解吸器的分子机制。提取蛋白经胰蛋白酶消化，LC分离，MS/MS分析。利用MS/MS数据检索自定义的Bgh EST序列数据库和NCBI-NR真菌蛋白数据库，鉴定出204个吸器蛋白。大多数蛋白质似乎在蛋白质代谢途径和生物能量产生中起作用。令人惊讶的是，参与酒精发酵的丙酮酸脱羧酶（PDC）在真菌和植物中普遍存在，但在我们的Bgh蛋白质组数据集中却没有出现。在我们的EST序列数据库中也没有编码该酶的序列。值得注意的是，BLAST搜索最近可用的Bgh基因组序列数据也未能识别编码该酶的序列，这强烈表明Bgh不具有PDC基因。
A number of fungal and oomycete plant pathogens of major economic importance feed on their hosts by means of haustoria, which they place inside living plant cells. The underlying mechanisms are poorly understood, partly due to difficulty in preparing haustoria. We have therefore developed a procedure for isolating haustoria from the barley powdery mildew fungus (Blumeria graminis f.sp. hordei, Bgh). We subsequently aimed to understand the molecular mechanisms of haustoria through a study of their proteome. Extracted proteins were digested using trypsin, separated by LC, and analysed by MS/MS. Searches of a custom Bgh EST sequence database and the NCBI-NR fungal protein database, using the MS/MS data, identified 204 haustoria proteins. The majority of the proteins appear to have roles in protein metabolic pathways and biological energy production. Surprisingly, pyruvate decarboxylase (PDC), involved in alcoholic fermentation and commonly abundant in fungi and plants, was absent in our Bgh proteome data set. A sequence encoding this enzyme was also absent in our EST sequence database. Significantly, BLAST searches of the recently available Bgh genome sequence data also failed to identify a sequence encoding this enzyme, strongly indicating that Bgh does not have a gene for PDC.