PSA-NCAM expression in the piriform cortex of the adult rat. Modulation by NMDA receptor antagonist administration
PSA-NCAM expression in the piriform cortex of the adult rat. Modulation by NMDA receptor antagonist administration
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DOI：
10.1016/s0006-8993(01)03241-3
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发表时间：
2002-02-15
期刊：
影响因子：
2.9
通讯作者：
McEwen, B
中科院分区：
文献类型：
--
作者：
Nacher, J;Alonso-Llosa, G;McEwen, B
NMDA受体拮抗剂的管理上调表达的聚唾液酸化形式的神经细胞粘附分子（PSA-NCAM）在成年海马。由于梨状皮质在成年期也被PSA-NCAM免疫反应性神经元占据，我们试图详细描述它们，并测试NMDA受体拮抗剂是否也调节PSA-NCAM在这个皮质区域。PSA-NCAM免疫反应主要位于第II层，其中许多神经胶质样细胞和一些延髓-半月过渡神经元被标记。第三层和内虹膜状核中的许多大神经元也表达PSA-NCAM。有趣的是，一些小的标记细胞类似于迁移的神经母细胞出现在这些层中，并在胼胝体腹侧端的梨状皮质。这些假定的迁移细胞和一些神经胶质样神经元在第二层不表达NeuN，分化的神经元的标记。这些PSA-NCAM免疫反应性细胞中的许多也表达doublecortin，一种参与神经元迁移的分子。在给予NMDA受体拮抗剂后7天，PSA-NCAM免疫反应性神经胶质样神经元的数量显著增加。此外，治疗后21天，我们观察到梨状皮质深层中表达doublecortin的细胞数量显著增加。这些结果扩大了目前的知识，在梨状皮质表达PSA-NCAM的神经元群体，这表明，这些细胞中的一些可能参与结构的可塑性事件，如轴突生长，突触，甚至神经元迁移。类似于海马，NMDA受体似乎在成人梨状皮质的这些过程中发挥关键作用。(C)2002 Elsevier Science B. V.保留所有权利。
Administration of NMDA receptor antagonists upregulates the expression of the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) in the adult hippocampus. Since the piriform cortex is also populated by PSA-NCAM immunoreactive neurons during adulthood, we sought to characterize them in detail and to test whether NMDA receptor antagonists also modulate PSA-NCAM in this cortical region. PSA-NCAM immunoreactivity is located mainly in layer II, where many neurogliaform and some pyramidal-semilunar transitional neurons are labeled. Many large neurons in layer III and endopiriform nucleus also express PSA-NCAM. Interestingly, some small labeled cells resembling migratory neuroblasts appear in these layers and in the ventral end of the corpus callosum subjacent to the piriform cortex. These putative migratory cells and some neurogliaform neurons in layer II do not express NeuN, a marker of differentiated neurons. Many of these PSA-NCAM immunoreactive cells also express doublecortin, a molecule involved in neuronal migration. The number of PSA-NCAM immunoreactive neurogliaform neurons increases significantly 7 days after the administration of an NMDA receptor antagonist. Moreover, 21 days after the treatment we observe a significant increase in the number of doublecortin expressing cells in the deep layers of the piriform cortex. These results expand the current knowledge of the neuronal populations expressing PSA-NCAM in the piriform cortex, suggesting that some of these cells could be involved in structural plastic events such as axonal outgrowth, synaptogenesis or even neuronal migration. Similar to the hippocampus, NMDA receptors appear to play a critical role in these processes in the adult piriform cortex. (C) 2002 Elsevier Science B.V. All rights reserved.