SEX-RELATED AND AGE-RELATED VARIATIONS IN THE INVITRO HEPARIN-RELEASABLE LIPOPROTEIN-LIPASE FROM MONONUCLEAR LEUKOCYTES IN BLOOD
SEX-RELATED AND AGE-RELATED VARIATIONS IN THE INVITRO HEPARIN-RELEASABLE LIPOPROTEIN-LIPASE FROM MONONUCLEAR LEUKOCYTES IN BLOOD
复制标题
DOI：
10.1016/0005-2760(90)90163-r
复制
发表时间：
1990-02-23
期刊：
影响因子：
--
通讯作者：
EGUCHI, Y
中科院分区：
文献类型：
--
作者：
MAEHIRA, F;MIYAGI, I;EGUCHI, Y
在全血（主要是单核细胞）中肝素释放脂蛋白脂肪酶（LPL）的实验证明：(1)在37℃条件下，10 U/ml肝素存在时的溶脂活性的时间过程，(2)LPL活性在单核细胞和淋巴细胞组分中的分布，(3)抗LPL免疫球蛋白（IgG）的免疫失活，(4)对各种化合物如NaCl、硫酸鱼精蛋白、肝素和血清激活剂的反应。正常血脂和高血脂家兔血液中肝素释放的LPL活性与肝素后血浆的LPL活性具有良好的相关性。人体研究揭示了134名正常受试者和24名高甘油三酯血症受试者血液中单核细胞的体外肝素释放LPL的性别和年龄相关变化：30岁以上正常女性的平均LPL活性显著高于相应的男性。在高甘油三酯血症组中，女性的LPL活性也高于男性，但并不显著。血液中单核细胞的体外肝素释放LPL活性与脂肪组织和肝后血浆的LPL活性相当，因此它反映了脂蛋白代谢。
An in vitro heparin release of lipoprotein lipase (LPL) from whole blood, mainly from monocytes, was demonstrated by (1) the time-course of lipolytic activity with the presence of 10 U/ml heparin at 37.degree.C, (2) the distribution of LPL activity in monocyte and lymphocyte fractions, (3) an immuno-inactivation with anti-LPL immunoglobulin (IgG) and (4) responses to various compounds such as NaCl, protamine sulfate, heparin, and serum activator. The in vitro heparin-releasable LPL activity from blood correlated well with the LPL activity of postheparin plasma obtained from both normolipidemic and hyperlipidemic rabbits. Studies in humans revealed sex- and age-related variations in the in vitro heparin-releasable LPL from monocytes in the blood of 134 normal subjects and 24 hypertriglyceridemic subjects: The mean LPL activity was significantly higher in normal females over the age of 30, than in the corresponding males. In the hypertriglyceridemic group, the LPL activity was also higher in females than in males, but it was not significant. The in vitro heparin-releasable LPL activity from monocytes in blood was comparable to the LPL activity derived from adipose tissue and postheparine plasma and thus it reflects lipoprotein metabolism.