Identification and characterization of the human MOG1 gene.
Identification and characterization of the human MOG1 gene.
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DOI：
10.1016/s0378-1119(01)00364-x
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发表时间：
2001-03
期刊：
影响因子：
3.5
通讯作者：
Kavita A. Marfatia;Michelle T. Harreman;P. Fanara;P. Vertino;A. Corbett
中科院分区：
文献类型：
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作者：
Kavita A. Marfatia;Michelle T. Harreman;P. Fanara;P. Vertino;A. Corbett
许多介导转运进出细胞核的蛋白质在整个进化过程中在结构和功能上都是保守的。在这里，我们描述了人类MOG 1基因的序列和特征。MOG 1基因最初在酿酒酵母中被鉴定为酵母核转运因子GSP 1（scRan）的条件等位基因的多拷贝抑制子（Oki和Nishimoto（1998）Proc.Natl. Acad. Sci. USA 95，15388-15393）。对表达序列标签数据库的搜索鉴定了一种推定的人类蛋白质，其与酵母蛋白质具有29%的同一性和47%的相似性。我们的实验表明，人类MOG 1信息在各种组织样本中表达。几个实验表明，人MOG 1蛋白结合酵母和人Ran，表明酵母和人MOG 1蛋白之间的功能保守。此外，hMOG 1a与scMOG 1一样，定位于整个细胞，但集中在细胞核内。与这些发现一致，hMOG 1a可以部分弥补酵母MOG 1缺失细胞中存在的生长缺陷。综上所述，我们的研究结果表明，MOG 1是一种进化上保守的Ran结合蛋白，可能在调节核蛋白运输中发挥作用。
Many of the proteins that mediate transport into and out of the nucleus have been structurally and functionally conserved throughout evolution. Here we describe the sequence and characterization of the human MOG1 gene. The MOG1 gene was originally identified in Saccharomycescerevisiae as a multi-copy suppressor of conditional alleles of the yeast nuclear transport factor, GSP1 (scRan) (Oki and Nishimoto (1998) Proc. Natl. Acad. Sci. USA 95, 15388–15393). A search of the expressed sequence tag database identified a putative human protein that is 29% identical and 47% similar to the yeast protein. Our experiments demonstrate that the human MOG1 message is expressed in a variety of tissue samples. Several experiments indicate that the human MOG1 protein binds to both yeast and human Ran suggesting functional conservation between the yeast and human MOG1 proteins. Furthermore, hMOG1a, like scMOG1, is localized throughout the cell but is concentrated within the nucleus. Consistent with these findings, hMOG1a can partially complement the growth defect present in yeast MOG1 deletion cells. Taken together, our findings suggest that MOG1 is an evolutionarily conserved Ran binding protein that could play a role in regulating nuclear protein trafficking.