Adventitious Shoot and Callus Formation in Vitro from Young Leaves of Melastoma affine
Adventitious Shoot and Callus Formation in Vitro from Young Leaves of Melastoma affine
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发表时间：
2009
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通讯作者：
G. Ma;Yong Li;G. Jiao;Xiao-ping Fu;Yourun Lin
中科院分区：
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作者：
G. Ma;Yong Li;G. Jiao;Xiao-ping Fu;Yourun Lin
该属约有100种，主要分布于南亚、太平洋和澳大利亚。中国有9种1变种。它们主要分布在长江以南地区。其中，大多数种属的植物都有不同的叶子和美丽的花朵，用于观赏和园艺目的。本研究建立了一套仿木麻黄离体繁殖体系。以离体小植株的幼叶为外植体，在含有1.0mg·L-1噻苯隆或1.0mg·L-1噻苯隆+0.5mg·L-1 6-苄基腺嘌呤（BA）的MS培养基上诱导出愈伤组织。此后，在相同的培养基上，不定芽直接从愈伤组织发育。不定芽在附加0.2mg·l BA +0.02mg·l NAA的增殖培养基上可产生丛生芽，在附加0.1mg·l IBA的1/2 MS培养基上可生根和再生植株。建立了一个高效的离体繁殖和植株再生体系。_____________________________________________________________________________________________________________
The genus Melastoma contains about 100 species and is distributed mainly in South Asia, the Pacific Ocean and Australia. In China, there are nine species and one variety. They are mainly distributed in areas South of the Changjiang River. Among these, most species in the genus Melastoma have varying foliage and beautiful flowers that are used for ornamental and horticultural purposes. In this study, we established an in vitro propagation system for Melastoma affine. Young leaf lobes from in vitro plantlets were induced to form callus on MS medium containing 1.0 mg.l thidiazuron or 1.0 mg.l thidiazuron in combination with 0.5 mg.l 6-benzyladenine (BA). Thereafter, adventitious shoots developed directly from callus on the same medium. The adventitious shoots can develop multiple-shoots on the propagation medium containing 0.2 mg.l BA + 0.02 mg.l NAA and root formation and plantlets regeneration were achieved on 1⁄2MS medium with 0.1 mg.l IBA. An efficient in vitro propagation and plant regeneration system was successfully established. _____________________________________________________________________________________________________________