Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag.
Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag.
复制标题
DOI：
10.1039/c0cc02432e
复制
发表时间：
2010-10
影响因子：
4.9
通讯作者：
K. Sadhu;S. Mizukami;Shuji Watanabe;K. Kikuchi
中科院分区：
文献类型：
--
作者：
K. Sadhu;S. Mizukami;Shuji Watanabe;K. Kikuchi
目标蛋白与转基因β-内酰胺酶融合，在生理条件下与探针发生反应，打破荧光团与猝灭剂之间的聚集相互作用。这种联盟分离技术是一种新的蛋白质标记技术，并在体外和活细胞成像研究中进行了探索。
The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.