Optimization Protocol for the Microwave-Assisted Extraction of Antioxidant Components from Pinus elliottii Needles Using Response Surface Methodology
Optimization Protocol for the Microwave-Assisted Extraction of Antioxidant Components from Pinus elliottii Needles Using Response Surface Methodology
复制标题
使用响应面法从湿地松针叶中微波辅助提取抗氧化成分的优化方案
DOI：
10.15376/biores.12.1.478-494
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发表时间：
2017-01-01
期刊：
影响因子：
1.5
通讯作者：
Peng, Wanxi
中科院分区：
文献类型：
--
作者：
Ouyang, Hui;Hou, Kun;Peng, Wanxi
采用Box-Behnken旋转设计响应面法（RSM）确定微波辅助提取湿地松针叶中抗氧化剂的最佳工艺条件。在三个水平（29个实验条件）下评估四个工艺变量：乙醇（50%，70%和90%），溶剂：溶质比（25:1,20:1和15:1），提取温度（60,70和80℃），超声波功率（100,150和200 W）。利用RSM法，通过多元回归分析得到二次多项式方程，预测了最佳提取方案。自由基清除能力由O-2（-）决定。OH和DPPH方法。微波辅助提取湿地松针叶中抗氧化物质的最佳工艺条件为：乙醇浓度为72%，溶剂：溶质比为21:1 mL/g，提取温度为67℃，超声功率为200 W。结果表明，总多酚含量与O-2、中心点OH和DPPH自由基清除能力有良好的相关性。
Response surface methodology (RSM) based on a Box-Behnken rotatable design was used to determine the optimum conditions for the microwave-assisted extraction of antioxidant compounds from Pinus elliottii needles. Four process variables were evaluated at three levels (29 experimental conditions): ethanol (50, 70, and 90%), solvent: solute ratio (25: 1, 20: 1, and 15: 1), extraction temperature (60, 70, and 80 degrees C), and ultrasonic power (100, 150, and 200 W). Using RSM, a quadratic polynomial equation was obtained by multiple regression analysis to predict the optimized extraction protocol. The radical scavenging capacity was determined by O-2(-),. OH, and DPPH methods. For the microwaveassisted extraction of antioxidant compounds from Pinus elliottii needles, the optimum process used ethanol at 72%, a solvent: solute ratio of 21: 1 mL/g, an extraction temperature of 67 degrees C, and an ultrasonic power of 200 W. The results indicated good correlation between total polyphenols content and O-2, center dot OH, and DPPH radical scavenging activities.