A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium

A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium
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诱导多能干细胞逐步分化为视网膜色素上皮的方案

DOI:
10.1007/7651_2021_418
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发表时间:
2021
期刊:
Methods Mol Biol
影响因子:
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通讯作者:
Tsujikawa Akitaka
Tsujikawa Akitaka
中科院分区:
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文献类型:
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作者:
Tagawa Miho;Ikeda Hanako Ohashi;Hata Masayuki;Inoue Yumi;Iwai Sachiko;Tsujikawa Akitaka

文献摘要

相似文献

我们已经建立了一种将诱导多能干细胞(iPSC)分化为视网膜色素上皮(RPE)(iPSC-RPE)的分步方法,该方法能够有效分离和纯化高质量的患者来源的iPSC-RPE细胞。在这里,我们详细描述了将iPSC分化为iPSC-RPE的过程。
We have established a stepwise method to differentiate induced pluripotent stem cells (iPSCs) into retinal pigment epithelium (RPE) (iPSC-RPE), which enables efficient isolation and purification of patient-derived iPSC-RPE cells with high quality. Here, we describe in detail the process of differentiating iPSCs into iPSC-RPE.