NEUTROPHIL ACTIVATION BY MONOMERIC INTERLEUKIN-8
NEUTROPHIL ACTIVATION BY MONOMERIC INTERLEUKIN-8
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DOI：
10.1126/science.8140420
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发表时间：
1994-04-01
期刊：
影响因子：
56.9
通讯作者：
CLARKLEWIS, I
中科院分区：
文献类型：
--
作者：
RAJARATHNAM, K;SYKES, BD;CLARKLEWIS, I
白细胞介素-8（IL-8）是一种促炎蛋白，核磁共振（NMR）和X射线技术已显示其以同源二聚体形式存在。化学合成IL-8类似物，其中亮氨酸-25的酰胺氮甲基化以选择性地阻断单体之间氢键的形成，从而防止二聚化。通过分析超速离心和核磁共振评估，该类似物被证明是单体。然而，在中性粒细胞活化测定中，它与IL-8相当，这表明单体是IL-8的功能形式。
Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers.and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.