Phosphorus-31 and carbon-13 nuclear magnetic resonance studies of divalent cation binding to phosphatidylserine membranes: use of cobalt as a paramagnetic probe.
Phosphorus-31 and carbon-13 nuclear magnetic resonance studies of divalent cation binding to phosphatidylserine membranes: use of cobalt as a paramagnetic probe.
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二价阳离子与磷脂酰丝氨酸膜结合的磷 31 和碳 13 核磁共振研究：使用钴作为顺磁探针。
DOI：
10.1021/bi00263a008
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
McLaughlin,AC
中科院分区：
文献类型：
--
作者：
McLaughlin,AC
材料和方法材料。牛脑磷脂酰丝氨酸（PS）购自Avanti Biochemicals（Birmingham，AL）。在其纯化程序的最后一步中，将其与0.5%乙二胺四乙酸（EDTA）分配，以除去二价阳离子和重金属杂质。通过用水洗涤三次除去残留的EDTA。氧化氘（99.8%）获自Bio-Rad Laboratories（里士满，CA），并且不经进一步处理而使用。氯化钠以超纯级购自马萨诸塞州丹弗斯的康龙公司（Danvers，MA）。所有其他无机化学品均为试剂级。2-（7V-吗啉代）乙磺酸（Mes）从PL Biochemicals，Inc.获得。（密尔沃基，WI）。三-（羟甲基）氨基甲烷（Tris）得自Sigma Chemical Co. (St. Louis，MO）和3-（7 N-吗啉代）丙磺酸（Mops）从Calbiochem（拉霍亚，CA）获得。通过使H2O通过Milli-Q离子交换系统（Millipore，贝德福德，MA）来纯化H2O，并且H2O具有小于10 - 7 - 1/2的电导率。31 P和13 C NMR测量。在Bruker WH-360光谱仪上分别在145.7和90.5 MHz下获得超声处理的PS囊泡的31 P和13 C NMR光谱。用于温度依赖性研究的溶液（见附录）含有15% 2 H2O，氘共振用作“锁定”。钴对横向
Materials and Methods Materials. Bovine brain phosphatidylserine (PS) was purchased from Avanti Biochemicals (Birmingham, AL). In the final step of their purification procedure, it was partitioned against 0.5% ethylenediaminetetraacetic acid (EDTA) to remove divalent cation and heavy metal impurities. Residual EDTA was removed by three washes with water. Deuterium oxide (99.8%) was obtained from Bio-Rad Laboratories (Richmond, CA) and used without further treatment. Sodium chloride was obtained from Ventrón (Danvers, MA) as the Ultrapure grade. All other inorganic chemicals were reagent grade. 2-(7V-Morpholino) ethanesulfonic acid (Mes) was ob-tained from PL Biochemicals, Inc.(Milwaukee, WI). Tris-(hydroxymethyl) aminomethane (Tris) was obtained from Sigma Chemical Co.(St. Louis, MO), and 3-(7V-morpholino) propanesulfonic acid (Mops) was obtained from Calbiochem (La Jolla, CA). H20 was purified by passage through a Milli-Q ion-exchange system (Millipore, Bedford, MA) and had a conductivity of less than 10~ 7 _1/2. 31P and 13C NMR Measurements. 31P and 13C NMR spectra of sonicated PS vesicles were obtained at 145.7 and 90.5 MHz, respectively, on a Bruker WH-360 spectrometer. The solution used for the temperature-dependence studies (see Appendix) contained 15% 2H20, and the deuterium resonance was used as a “lock”. The effect of cobalt on the transverse