Post-transcriptional control of human cytomegalovirus gene expression.
Post-transcriptional control of human cytomegalovirus gene expression.
复制标题
人类巨细胞病毒基因表达的转录后控制。
DOI：
10.1016/0042-6822(83)90355-0
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发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
DeMarchi,JM
中科院分区：
文献类型：
--
作者：
DeMarchi,JM
在人类成纤维细胞中人类巨细胞病毒感染的早期、早期和晚期阶段，基因组上大约 20%、75% 和 90% 的序列分别积累了转录本。然而，并非所有在立即早期和早期阶段积累的序列都在多核糖体上出现。详细研究了放线菌酮处理的细胞中合成的四种转录本。 2.2-kb 转录本（713-733 个图谱单位）代表放线菌酮处理细胞中 95% 的多核糖体相关 RNA，并且是第一个在感染后 2 小时在未处理细胞中在多核糖体上检测到的转录物。在放线菌酮处理的细胞的多核糖体上还发现了第二个丰度较低的 5.2 kb（670-733 个图谱单位）转录本，初步证据表明该转录本可能在处理过程中被剪接。 3.25 kb 转录本（190-217 个图谱单位）也被鉴定为一种较小的多核糖体相关 RNA 种类。一个 4.8 kb (630-670) 的转录物仍然与细胞核相关，并且在放线菌酮处理的细胞中没有被加工成 mRNA。观察到不同转录本之间的差异稳定性，2.2-kb 转录本最稳定。结果表明，在人巨细胞病毒感染的细胞中，控制存在于转录物积累、转运到细胞质、优先与多核糖体结合以及RNA的相对稳定性水平上。
During the immediate-early, early, and late phases of human cytomegalovirus infection in human fibroblasts, transcripts accumulated, respectively, from approximately 20, 75, and 90% of the sequences on the genome. Not all of the sequences which accumulated during the immediate-early and early phases were represented on polysomes, however. Four transcripts synthesized in cycloheximide-treated cells were studied in detail. A 2.2-kb transcript (713–733 map units) represented 95% of the polysome-associated RNA in cycloheximide-treated cells and was the first to be detected on polysomes at 2 hr postinfection in untreated cells. A second, less abundant, transcript of 5.2 kb (670–733 map units) was also found on polysomes in cycloheximide-treated cells, and preliminary evidence suggested that this transcript may be spliced during processing. A 3.25-kb transcript (190–217 map units) was identified also as a minor polysome-associated species of RNA. One transcript of 4.8 kb (630–670) remained associated with the nucleus and was not processed into mRNA in cycloheximide-treated cells. Differential stability between the various transcripts was observed, the 2.2-kb transcript being the most stable. The results showed that in human cytomegalovirus-infected cells controls exist at the level of transcript accumulation, transport into the cytoplasm, preferential association with polysomes, and relative stability of RNAs.