Rat Hindlimb Cryopreservation and Transplantation: A Step Toward "Organ Banking"
Rat Hindlimb Cryopreservation and Transplantation: A Step Toward "Organ Banking"
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DOI：
10.1111/ajt.14320
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发表时间：
2017-11-01
影响因子：
8.8
通讯作者：
Shani, N.
中科院分区：
文献类型：
--
作者：
Arav, A.;Friedman, O.;Shani, N.
2016年，美国进行了超过500万次重建手术。最近临床血管化复合异体移植的成功，包括手部和面部移植，确立了这些增强生命重建的巨大潜力。然而，由于有限的可用性和终身免疫抑制，应用是有限的。复合移植的长期储存可能增加部分或完全HLA匹配的美观兼容部件的可用性，降低排斥反应的风险和免疫抑制负担。本研究的目的是制定有效的冷冻保存和移植完整的啮齿动物肢体的方案。定向冷冻是一种以等速线性温度梯度冷却样品的方法，可以精确控制过程和冰晶的形成。玻璃化是另一种冷冻保存方法，其中样品凝固而不形成冰晶。在大鼠后肢复合组织移植模型上对两种方法进行了测试，发现了可靠、可重复、稳定的复合组织保存方法。我们相信，随着进一步的研究和发展，冷冻保存可能会导致复合组织“银行”。这可能会导致一种范式的转变，从少数和遥远的紧急手术到大规模的、精心策划的、更好控制的选择性手术。
In 2016, over 5 million reconstructive procedures were performed in the United States. The recent successes of clinical vascularized composite allo-transplantations, hand and face transplantations included, established the tremendous potential of these life-enhancing reconstructions. Nevertheless, due to limited availability and lifelong immunosuppression, application is limited. Longterm banking of composite transplants may increase the availability of esthetically compatible parts with partial or complete HLA matching, reducing the risk of rejection and the immunosuppressive burden. The study purpose was to develop efficient protocols for the cryopreservation and transplantation of a complete rodent limb. Directional freezing is a method in which a sample is cooled at a constant-velocity linear temperature gradient, enabling precise control of the process and ice crystal formation. Vitrification is an alternative cryopreservation method in which the sample solidifies without the formation of ice crystals. Testing both methods on a rat hindlimb composite tissue transplantation model, we found reliable, reproducible, and stable ways to preserve composite tissue. We believe that with further research and development, cryopreservation may lead to composite tissue "banks." This may lead to a paradigm shift from few and far apart emergent surgeries to wide-scale, well-planned, and better-controlled elective surgeries.