Variables in the high-performance anion-exchange chromatography of proteins.
Variables in the high-performance anion-exchange chromatography of proteins.
复制标题
蛋白质高效阴离子交换色谱中的变量。
DOI：
10.1016/0003-2697(80)90658-2
复制
发表时间：
1980
影响因子：
2.9
通讯作者：
Regnier,FE
中科院分区：
文献类型：
--
作者：
Vanecek,G;Regnier,FE
考察了流动相速度、分离时间、载体孔径、柱长和温度对新型商用高效阴离子交换载体SynChropak AX-300的拆分和负载能力的影响。该材料是一种粒径为10μm的大孔球形二氧化硅，带有粘结的聚胺层。研究发现，卵清蛋白样品与牛血清白蛋白结合的异质性使其成为评价阴离子交换载体的有用探针。在4.1mmI.D.色谱柱中，当流速为0.25ml/min时，蛋白质的分离度最高。在4.1×250 mm AX-300色谱柱上，每次进样最多10 mg蛋白质，具有良好的分离度。长度为50 mm的色谱柱的蛋白质载量是250 mm色谱柱的十分之一，保留了大约75%的分辨率。
The effect of mobile phase velocity, separation time, support pore diameter, column length, and temperature on resolution and loading capacity of a new commercially available high-performance anion-exchange support, SynChropak AX-300, has been examined. This material is a macroporous spherical silica of 10 μm particle size with a bonded polymeric amine layer. It was found that the heterogeneity of ovalbumin samples, combined with bovine serum albumin, make them useful probes in evaluation of anion-exchange supports. In the columns of 4.1 mm i.d., the highest resolutions of proteins were achieved at a flow rate of 0.25 ml/min. Up to 10 mg of protein per injection could be applied on a 4.1 × 250 mm AX-300 column with good resolution. Columns of 50 mm length had one-tenth the protein load capacity of a 250-mm column, retaining approximately 75% of the resolution.