Developmental fate of pole cells in Drosophila melanogaster.
Developmental fate of pole cells in Drosophila melanogaster.
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DOI：
10.1016/0012-1606(80)90476-5
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发表时间：
1980-06
影响因子：
2.7
通讯作者：
E. M. Underwood;J. Caulton;C. Allis;A. Mahowald
中科院分区：
文献类型：
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作者：
E. M. Underwood;J. Caulton;C. Allis;A. Mahowald
极细胞除了是原始生殖细胞的前体之外，还被认为产生卵黄噬菌体和/或中肠上皮细胞。这项研究重新审视了果蝇中这些细胞的潜在命运。在移植放射性标记的极细胞后，使用形态学标准和放射自显影术追踪极细胞的运动。性腺形成完成后，大多数极细胞出现在性腺中。一些极细胞进入卵黄团，但它们完全封闭在卵黄膜内。这些细胞不会变成卵黄噬菌体，随后可能会退化。发现极细胞与性腺附近组织松散相关的比例较低。极细胞也存在于后肠腔、肛门开口和胚胎外部。我们认为，尽管不到一半形成（或注射）的极细胞最终到达性腺，但极细胞没有其他主要命运。 “额外”的极细胞要么被困在邻近组织中，要么在卵黄中退化，要么被排出体外。
Pole cells, in addition to being precursors of the primordial germ cells, have been thought to give rise to vitellophages and/or midgut epithelial cells. This study presents a reexamination of the potential fates of these cells inDrosophila melanogaster. Pole cell movements have been traced using both morphological criteria and autoradiography following transplantation of radioactively labeled pole cells. After the completion of gonad formation most pole cells are found in the gonads. Some pole cells enter the yolk mass, but these are completely enclosed within the yolk membrane. These cells do not become vitellophages and may later degenerate. A low percentage of pole cells are found loosely associated with tissues near the gonads. Pole cells are also found in the hindgut lumen, anal opening, and exterior of the embryo. We propose that, although less than half of the pole cells formed (or injected) ultimately reach the gonads, there is no other major fate for the pole cells. The “extra” pole cells either get trapped in neighboring tissues, degenerate in the yolk, or are excreted.