Cloning and tissue distribution of a new rat olfactory receptor-like (OL2)
Cloning and tissue distribution of a new rat olfactory receptor-like (OL2)
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DOI：
10.1006/bbrc.1997.8041
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发表时间：
1998-01-26
影响因子：
3.1
通讯作者：
Bataille, D
中科院分区：
文献类型：
--
作者：
Blache, P;Gros, L;Bataille, D
用聚合酶链式反应(PCR)克隆了G蛋白偶联受体超家族中一个新成员(命名为OL2)的无内含子基因。大鼠OL2受体基因的编码区预测了一个由315个氨基酸组成的7个跨膜区受体。OL2与在发育中的大鼠心脏表达的嗅觉受体OL1的氨基酸同源性为46.4%，与其他几种嗅觉受体的同源性略低。PCR分析表明，该转录本主要存在于大鼠脾和小鼠胰岛素分泌细胞系(MIN)中。OL2的组织分布与嗅觉相关的GTP结合蛋白Golfα亚基的分布没有相关性。这些发现表明，这种新的G蛋白偶联受体及其未知的配体在脾和分泌胰岛素的β细胞中发挥了作用。(C)1998年学术出版社。
Polymerase chain reaction (PCR) was used to clone an intronless cDNA encoding a new member (named OL2) of the G protein-coupled receptor superfamily. The coding region of the rat OL2 receptor gene predicts a seven transmembrane domain receptor of 315 amino acids. OL2 has 46.4 percent amino acid identity with OL1, an olfactory receptor expressed in the developing rat heart, and slightly lower percent indentities with several other olfactory receptors. PCR analysis reveals that the transcript is present mainly in the rat spleen and in a mouse insulin-secreting cell line (MING). No correlation was found between the tissue distribution of OL2 and that of the olfaction-related GTP-binding protein Golf alpha subunit. These findings suggest a role for this new hypothetical G-protein coupled receptor and for its still unknown ligand in the spleen and in the insulin-secreting beta cells. (C) 1998 Academic Press.