2,4-EPIBRASSIONOLIDE ACTIVATES PRIMING RESISTANCE AGAINST RHIZOPUS STOLONIFER INFECTION IN PEACH FRUIT
2,4-EPIBRASSIONOLIDE ACTIVATES PRIMING RESISTANCE AGAINST RHIZOPUS STOLONIFER INFECTION IN PEACH FRUIT
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2,4-表铜醇内酯可激活桃果实中针对根霉感染的引发抗性
DOI：
10.1556/066.2020.49.2.2
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发表时间：
2020-06-01
期刊：
影响因子：
1.1
通讯作者：
Wang, K. T.
中科院分区：
文献类型：
--
作者：
Li, C. H.;Du, M. Y.;Wang, K. T.
研究了2，4-表油菜素内酯(EBR)对桃采后根霉腐烂的抑制作用及其对果实生化防御反应的影响。在所有处理中，5亩M的EBR处理对桃果实根霉腐烂的抑制效果最佳。ERR处理显著上调了PpCHI、PpGns1、PpPAL、PpNPR1、PpPR1和PpPR4等一系列防御相关酶和PR基因的表达水平，并促进了桃树体内酚类和木质素的生物合成。有趣的是，EBR处理的桃树在接种病原菌后PR基因的表达和抗真菌化合物的积累更加显著，表明EBR可以激活引发防御。另一方面，5MU的EBR在体外对匍匐茎真菌的增殖有直接的毒性作用。由此可见，5亩M EBR对桃果实根霉腐烂病的抑制作用可能是通过直接抑制病原菌生长和间接诱导抗根霉菌的作用实现的。这些发现为桃采后保鲜过程中真菌感染的控制提供了一种潜在的替代方案。
This study was conducted to assess the effects of 2,4-epibrassionolide (EBR) on mold decay caused by Rhizopus stolonifer and its capability to activate biochemical defense reactions in postharvest peaches. The treatment of EBR at 5 mu M possessed the optimum effectiveness on inhibiting the Rhizopus rot in peach fruit among all treatments. The ERR treatment significantly up-regulated the expression levels of a set of defense-related enzymes and PR genes that included PpCHI, PpGns1, PpPAL, PpNPR1, PpPR1 and PpPR4 as well as led to an enhancement for biosynthesis of phenolics and lignins in peaches during the incubation at 20 degrees C. Interestingly, the EBR-treated peaches exhibited more striking expressions of PR genes and accumulation of antiftungal compounds upon inoculation with the pathogen, indicating a priming defense could be activated by EBR. On the other hand, 5 mu M EBR exhibited direct toxicity on fungal proliferation of R. stolonifer in vitro. Thus, we concluded that 5 mu M EBR inhibited the Rhizopus rot in peach fruit probably by a direct inhibitory effect on pathogen growth and an indirect induction of a priming resistance. These findings provided a potential alternative for control of fungal infection in peaches during the postharvest storage.