PDE1 encodes a P-type ATPase involved in appressorium-mediated plant infection by the rice blast fungus Magnaporthe grisea
PDE1 encodes a P-type ATPase involved in appressorium-mediated plant infection by the rice blast fungus Magnaporthe grisea
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DOI：
10.1105/tpc.13.9.1987
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发表时间：
2001-09-01
期刊：
影响因子：
11.6
通讯作者：
Talbot, NJ
中科院分区：
文献类型：
--
作者：
Balhadère, PV;Talbot, NJ
稻瘟病菌对植物的感染是由一种叫做附着胞的特殊感染细胞引起的。这些感染细胞产生巨大的膨压，这是翻译成一个入侵的力量，允许一个狭窄的渗透菌丝突破植物角质层。Magnaporthe pde1突变体先前通过限制性内切酶介导的DNA整合诱变鉴定，并且其阐述穿透菌丝的能力受损。在这里，我们报告的pde1突变是插入到启动子的P型ATP酶编码基因的结果。靶向基因破坏证实了PDE1在穿透菌丝发育和致病性中的作用，但强调了不同稻瘟病菌菌株中PDE1调控的潜在差异。预测的PDE1基因产物与P型ATP酶的氨基磷脂转位酶组的成员最相似，并且被证明是酵母ATP酶基因ATC8的功能同源物。空间表达研究表明，PDE1在萌发的分生孢子和发育中的附着胞中表达。这些发现暗示氨基磷脂移位酶在穿透菌丝的发展和真菌在第一表皮细胞定植后的增殖中的作用。
Plant infection by the rice blast fungus Magnaporthe grisea is brought about by the action of specialized infection cells called appressoria. These infection cells generate enormous turgor pressure, which is translated into an invasive force that allows a narrow penetration hypha to breach the plant cuticle. The Magnaporthe pde1 mutant was identified previously by restriction enzyme-mediated DNA integration mutagenesis and is impaired in its ability to elaborate penetration hyphae. Here we report that the pde1 mutation is the result of an insertion into the promoter of a P-type ATPase-encoding gene. Targeted gene disruption confirmed the role of PDE1 in penetration hypha development and pathogenicity but highlighted potential differences in PDE1 regulation in different Magnaporthe strains. The predicted PDE1 gene product was most similar to members of the aminophospholipid translocase group of P-type ATPases and was shown to be a functional homolog of the yeast ATPase gene ATC8. Spatial expression studies showed that PDE1 is expressed in germinating conidia and developing appressoria. These findings implicate the action of aminophospholipid translocases in the development of penetration hyphae and the proliferation of the fungus beyond colonization of the first epidermal cell.