Pancreatic islet discrimination of hexose anomers. II. Transient computer simulation.
Pancreatic islet discrimination of hexose anomers. II. Transient computer simulation.
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己糖端基异构体的胰岛辨别。
DOI：
10.1152/ajpendo.1988.255.2.e201
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
Garfinkel,D
中科院分区：
文献类型：
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作者：
Achs,MJ;Garfinkel,D
我们之前在理想的稳态条件下模拟了胰岛糖酵解，其中输入是纯己糖异头物，没有突变，并重现了已知的对葡萄糖和甘露糖α异头物作为底物的偏好。这个模型在这里被扩展到模拟真实的实验，在实验中，六糖体会发生变异，并且随着时间的推移可以进行测量。模型系统的行为与现有的实验数据一致。磷酸果糖激酶的己糖二磷酸激活剂，其作用在之前的稳态分析中被认为不重要，在这里发现对其通量有适度的影响（大约10-15%）。在实际实验中，糖酵解途径的头型偏好遵循葡萄糖激酶的头型偏好的结论仍然成立。
We have previously modeled pancreatic islet glycolysis under idealized steady-state conditions where the input is a pure hexose anomer and there is no mutarotation and reproduced the known preference for the alpha-anomers of glucose and mannose as substrates. This model is here extended to simulate real experiments, where the hexoses mutarotate and measurements may be taken over time. The behavior of our model system agrees with available experimental data. The hexose diphosphate activators of phosphofructokinase, whose effect was seen as not important in the preceding steady-state analysis, are found here to have a modest (approximately 10–15%) effect on its flux. The previous conclusion that the anomeric preference of the glycolytic pathway follows from that of glucokinase continues to hold in the real experimental situation.