Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli
Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli
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DOI：
10.1128/aem.63.4.1428-1433.1997
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发表时间：
1997-04-01
影响因子：
4.4
通讯作者：
Aono, R
中科院分区：
文献类型：
--
作者：
Asako, H;Nakajima, H;Aono, R
我们之前报道过 soxS 或 robA 基因的过表达导致几种大肠杆菌菌株获得更高的有机溶剂耐受性并且还增加了对多种抗生素的耐药性（H. Nakajima、K. Kobayashi、M. Kobayashi、H. Asako 和 R. Aono, Appl. Environ, Microbiol. 61:2302-2307, 1995)。大多数大肠杆菌菌株在环己烷存在下无法生长。我们从 Kohara lambda 噬菌体克隆和环己烷耐受突变株 OST3408 中分离出 marRAB 基因。我们发现OST3408的marR编码区73位处的精氨酸被丝氨酸取代，并命名为基因marR08，我们的遗传分析表明marR08负责环己烷耐受表型，我们观察到高拷贝数质粒上的marA基因增加了大肠杆菌菌株的有机溶剂耐受性，此外，将大肠杆菌细胞暴露于水杨酸盐，这也激活了mar调节子基因，提高有机溶剂耐受性，marA、soxS 或 robA 基因的过度表达增加了对多种抗生素的耐药性，但不增加对亲水性氨基糖苷类的耐药性。
We previously reported that overexpression of the soxS or robA gene causes in several Escherichia coli strains the acquisition of higher organic solvent tolerance and also increased resistance to a number of antibiotics (H. Nakajima, K. Kobayashi, M. Kobayashi, H. Asako, and R. Aono, Appl. Environ, Microbiol. 61:2302-2307, 1995). Most B. coli strains cannot grow in the presence of cyclohexane. We isolated the marRAB genes from a Kohara lambda phage clone and cyclohexane-tolerant mutant strain OST3408. We found a substitution of serine for arginine at position 73 in the coding region of marR of OST3408 and designated the gene marR08, Our genetic analysis revealed that marR08 is responsible for the cyclohexane-tolerant phenotype, We observed that the marA gene on high-copy-number plasmids increased the organic solvent tolerance of E. coli strains, Furthermore, exposure of E. coli cells to salicylate, which activates the mar regulon genes, also raised organic solvent tolerance, Overexpression of the marA, soxS, or robA gene increased resistance to numerous antibiotics but not to hydrophilic aminoglycosides.