High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation
High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation
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DOI：
10.1016/s1044-0305(02)00351-3
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发表时间：
2002-04-01
影响因子：
3.2
通讯作者：
Costello, CE
中科院分区：
文献类型：
--
作者：
O'Connor, PB;Mirgorodskaya, E;Costello, CE
在基质辅助激光解吸/电离傅里叶变换质谱（MALDI-FTMS）源中瞬时升高压力至1-10 mbar范围内，降低了神经节苷脂的亚稳态碎裂。这允许在不损失高度不稳定的唾液酸残基的情况下检测分子离子种类。在这些实验中，神经节苷脂与多达五个唾液酸的MALDI电离和FTMS检测。在每种情况下，当使用高压碰撞冷却时，单电荷分子离子是光谱中的基峰，无论是在正离子模式还是负离子模式下，并且观察到最小的亚稳态碎裂。这一结果是有希望的，因为先前开发的TLC分离方法可以耦合到MALDI-FTMS。(C)2002年美国质谱学会。
Transiently elevating pressure in a matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) source into the 1-10 mbar range during ionization decreases the metastable fragmentation of gangliosides. This allows detection of the molecular ion species without loss of the highly labile sialic acid residues. In these experiments, gangliosides with up to five sialic acids were ionized by MALDI and detected with the FTMS. In each case, when the high pressure collisional cooling was used, the singly charged molecular ion was the base peak in the spectra, both in the positive and negative ion modes, and minimal metastable fragmentation was observed. This result is promising, as the previously developed TLC separation methods can be coupled to MALDI-FTMS. (C) 2002 American Society for Mass Spectrometry.