Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades
Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades
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DOI：
10.1002/cssc.201200365
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发表时间：
2012-01-01
期刊：
影响因子：
8.4
通讯作者：
Sieber, Volker
中科院分区：
文献类型：
--
作者：
Guterl, Jan-Karl;Garbe, Daniel;Sieber, Volker
由于化石资源的供应有限，需要开发可再生的石油产品替代品。在这里，生物基高级醇如异丁醇是用于合成化学商品和燃料的通用平台分子。目前，它们的基于发酵的生产受到微生物生产系统对终产物的低耐受性以及进入细胞代谢的低底物通量的限制。我们开发了一种创新的无细胞方法，利用人工最小化的糖酵解反应级联，只需要一个单一的辅酶。使用该工具箱实现了从葡萄糖无细胞生产乙醇和异丁醇。我们还证实，这些流线型级联在微生物生产停止的条件下发挥作用。我们的系统可以扩展到一系列工业相关的分子。耐溶剂生物催化剂的应用潜在地允许高产物产率，这显著地简化了下游产物回收。
The limited supply of fossil resources demands the development of renewable alternatives to petroleum-based products. Here, biobased higher alcohols such as isobutanol are versatile platform molecules for the synthesis of chemical commodities and fuels. Currently, their fermentation-based production is limited by the low tolerance of microbial production systems to the end products and also by the low substrate flux into cell metabolism. We developed an innovative cell-free approach, utilizing an artificial minimized glycolytic reaction cascade that only requires one single coenzyme. Using this toolbox the cell-free production of ethanol and isobutanol from glucose was achieved. We also confirmed that these streamlined cascades functioned under conditions at which microbial production would have ceased. Our system can be extended to an array of industrially-relevant molecules. Application of solvent-tolerant biocatalysts potentially allows for high product yields, which significantly simplifies downstream product recovery.