A comparative proteomic approach to identify defence-related proteins between resistant and susceptible rice cultivars challenged with the fungal pathogen Rhizoctonia solani
A comparative proteomic approach to identify defence-related proteins between resistant and susceptible rice cultivars challenged with the fungal pathogen Rhizoctonia solani
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一种比较蛋白质组学方法，用于鉴定受到真菌病原体立枯丝核菌挑战的抗性和易感水稻品种之间的防御相关蛋白
DOI：
10.1007/s10725-019-00551-w
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发表时间：
2019-10-22
影响因子：
4.2
通讯作者：
Niu, Dongdong
中科院分区：
文献类型：
--
作者：
Ma, Hongyu;Sheng, Cong;Niu, Dongdong
水稻纹枯病是由立枯丝核菌（Rhizoctoniasolani）引起的一种世界性水稻病害，其寄主免疫的分子机制尚不清楚。本研究以特青（抗病）和Lemont（感病）两个水稻品种为材料，对接种稻瘟病菌后的蛋白质组进行了比较分析。使用八重iTRAQ（isobaric tags for relative and absolute quantitation）技术进行solani，导致6560种蛋白质的鉴定和定量。共检测到755个蛋白质在不同侵染程度的植株中表达量发生了显著变化。基于误差因子< 2和大于1.5倍或小于0.67倍的数量差异，茄属植物控制植物。差异蛋白质主要涉及乙醛酸和二羧酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、不饱和脂肪酸生物合成和糖酵解/糖异生调节途径（p < 0.01）。此外，通过qRT-PCR检测了编码所选蛋白的基因的表达水平，并通过农杆菌渗入法检测了其在本氏烟草中的功能。基于这些蛋白质组学和实验数据，推测了水稻免疫受R。提出了Solaniinfection。本研究中鉴定的蛋白质为阐明水稻抗稻瘟病菌侵染的分子机制奠定了基础。索拉尼
Rice sheath blight, caused byRhizoctonia solani, is a major worldwide rice disease for which little is known about the molecular mechanisms of host immunity to infection. In the present study, a comparative proteomic analysis of two rice cultivars, Teqing (resistant) and Lemont (susceptible), inoculated withR. solaniwas conducted using an eight-plex iTRAQ (isobaric tags for relative and absolute quantitation) technique, resulting in the identification and quantification of 6560 proteins. A total of 755 proteins showed significant changes in abundance between plants infected withR. solaniand control plants, based on an error factor < 2 and a more than 1.5-fold or less than 0.67-fold quantitative difference. The differentially abundant proteins were mainly involved in glyoxylate and dicarboxylate metabolism; glycine, serine and threonine metabolism; unsaturated fatty acid biosynthesis; and glycolysis/gluconeogenesis regulation pathways (p < 0.01). In addition, the expression levels of the genes encoding selected proteins were tested by qRT-PCR, and their functions were tested inNicotiana benthamianavia agroinfiltration. Based on these proteomic and experimental data, a putative model of the regulation of rice immunity underR. solaniinfection is proposed. The proteins identified in the present study provide a basis for elucidating the molecular mechanisms underlying rice immunity to infection byR. solani.