Environment-Dependent Self-Assembly of DNA Origami Lattices on Phase-Separated Lipid Membranes
Environment-Dependent Self-Assembly of DNA Origami Lattices on Phase-Separated Lipid Membranes
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DOI：
10.1002/admi.201800437
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发表时间：
2018-07-23
影响因子：
5.4
通讯作者：
Suzuki, Yuki
中科院分区：
文献类型：
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作者：
Sato, Yusuke;Endo, Masayuki;Suzuki, Yuki
设计的纳米结构在脂质膜上的自组装为增强其表面的功能提供了一种强有力的方法。在这里，钝端DNA折纸结构的环境依赖组装在由液体无序（Ld）和固体有序（So）相组成的相分离的脂质双层膜上。荧光显微镜和原子力显微镜（AFM）成像显示，DNA折纸结构优先结合到So相。在较高浓度下，钝端折纸通过表面介导的自组装在Ld相上形成二维晶格。相反，那些吸附在So相上的分子流动性较差，聚集成聚集体。然而，加入NaCl改变了这种情况。高速AFM成像揭示了nacl诱导变化的机制，其中Ld相上的晶格从表面解吸，而So相上的堆积聚集体重新组织成晶格。这些结果表明，二维晶格的形成取决于脂质的相；然而，它可以通过离子条件来调节，使我们能够选择晶格形成的区域。目前的结果可能指导创建膜支持的DNA纳米结构，以脂质相或离子响应的方式自组装成功能状态。
Self-assembly of designed nanostructures on a lipid membrane offers a powerful approach for enhancing the functionality of its surface. Here, the environment-dependent assembly of blunt-ended DNA origami structures on a phase-separated lipid-bilayer membrane consisting of liquid-disordered (Ld) and solid-ordered (So) phases is reported. Fluorescence microscopy and atomic force microscopy (AFM) imaging reveal that DNA origami structures preferentially bind to the So phase. At higher concentrations, blunt-ended origamis form 2D lattices on the Ld phase through surface-mediated self-assembly. In contrast, those adsorbed on the So phase are less mobile and pack into aggregates. However, this situation is changed by adding NaCl. High-speed AFM imaging reveals the mechanism underlying the NaCl-induced changes, wherein the lattices on the Ld phase desorb from the surface while the packed aggregates on the So phase reorganize into the lattices. These results indicate that the formation of 2D lattices depends on the phase of the lipids; however, it can be tuned by ionic conditions, enabling us to select the domain on which the lattice is formed. The present results may guide the creation of membrane-supported DNA nanostructures that self-assemble into functional states in a lipid phase or ion-responsive manner.