INVITRO INCORPORATION OF SELENOMETHIONINE INTO PROTEIN BY VIGNA-RADIATA POLYSOMES
INVITRO INCORPORATION OF SELENOMETHIONINE INTO PROTEIN BY VIGNA-RADIATA POLYSOMES
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DOI：
10.1104/pp.66.1.182
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发表时间：
1980-01-01
期刊：
影响因子：
7.4
通讯作者：
SHRIFT, A
中科院分区：
文献类型：
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作者：
EUSTICE, DC;FOSTER, I;SHRIFT, A
辐射藤V.radiata（L.）Wilczek多聚体在体外有效地掺入[75 Se]硒代蛋氨酸、[14 C]蛋氨酸和[14 C]亮氨酸。翻译的最佳条件被确定为4.8 mM Mg2+，182 mM K+和pH 7.4。当分别测量时，[75 Se]硒代蛋氨酸和[14 C]甲硫氨酸的掺入率相似，但当两种氨基酸以等摩尔浓度存在时，[75 Se]硒代蛋氨酸掺入比[14 C]甲硫氨酸掺入低35%。热三氯乙酸可沉淀的翻译产物的聚丙烯酰胺凝胶电泳证明在[75 Se]硒代蛋氨酸或[35 S]蛋氨酸存在下合成高MW标记的蛋白质。在电泳图谱中未检测到MW的重大差异。在V.radiata多聚核糖体翻译过程中硒代蛋氨酸的利用为易受硒毒性影响的植物同化硒代蛋氨酸建立了途径。
V. radiata (L.) Wilczek polysomes efficiently incorporated [75Se]selenomethionine, [14C]methionine, and [14C]leucine in vitro. The optimal conditions for translation were determined to be 4.8 mM Mg2+, 182 mM K+ and pH 7.4. The rates of incorporation of [75Se]selenomethionine and [14C]methionine were similar when measured separately, but [75Se]selenomethionine incorporation was 35% less than [14C]methionine incorporation when both amino acids were present in equal molar concentrations. Polyacrylamide gel electrophoresis of the hot trichloroacetic acid precipitable translation products demonstrated synthesis of high MW labeled proteins in the presence of [75Se]selenomethionine or [35S]methionine. No major differences in MW could be detected in the electrophoretic profiles. Utilization of selenomethionine during translation by V. radiata polysomes establishes a route for the assimilation of selenomethionine by plants susceptible to selenium toxicity.