Partial cloning and distribution of estrogen receptor beta in the avian brain
Partial cloning and distribution of estrogen receptor beta in the avian brain
复制标题
禽脑中雌激素受体β的部分克隆和分布
作者：
B. Lakaye;AgnèAs Foidart;T. Grisar;J. Balthazart
采用RT-PCR技术从鹌鹑睾丸RNA中分离出雌激素受体β (ER-β)部分cDNA。RT-PCR结果显示，ER-β在雄性鹌鹑的端脑、间脑、垂体、睾丸和肾脏中高表达，而在小脑、胸肌和肾上腺中低表达或不表达。用35s标记的寡聚探针原位杂交通过下丘脑吻侧视前区切片，在内侧视前核、床终纹核和带核中发现高表达。这些数据表明，在非哺乳动物物种中，ER-β存在于与生殖控制有关的大脑区域。
A partial estrogen receptor beta (ER-β) cDNA was isolated from testicular quail RNA by RT-PCR with degenerate primers specific to the rat ER-β sequence. A high expression of ER-β was demonstrated by RT-PCR in the telencephalon, diencephalon, pituitary, testis and kidneys of male quail but little or no expression was detected in the cerebellum, pectoral muscle and adrenal gland. In situ hybridization with a 35S-labelled oligoprobe in sections through the preoptic area-rostral hypothalamus identified high expression in the medial preoptic nucleus, bed nucleus striae terminalis and nucleus taeniae. These data demonstrate the presence of an ER-β in brain areas implicated in the control of reproduction in a non-mammalian species.