First report and genetic characterization of porcine astroviruses of lineage 4 and 2 in diarrhoeic pigs in India
First report and genetic characterization of porcine astroviruses of lineage 4 and 2 in diarrhoeic pigs in India
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DOI：
10.1111/tbed.13058
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发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Singh, Raj Kumar
中科院分区：
文献类型：
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作者：
Kattoor, Jobin Jose;Malik, Yashpal Singh;Singh, Raj Kumar
猪星状病毒（Porcine astroviruses，PAstV）已在全球范围内广泛分布并具有很高的流行率;然而，其临床意义仍在研究中。到目前为止，关于它们在印度猪种群中的流行率和多样性的信息尚不清楚。这项研究是第一份关于印度仔猪中PAstV的患病率和遗传特征的报告。从2013年1月至2017年12月，使用RT-PCR检测筛选了757份样品，在17.6%（133/757）的猪中检测到PAstV感染。在133份阳性样品中，79份（59.4%）为PAstV单独阳性，而54份（40.6%）被发现与猪轮状病毒A（PoRVA）共感染。RdRp/衣壳基因区的系统发育分析显示PAstV序列之间具有高度的遗传异质性，PAstV谱系4占优势，谱系2的检测。在核苷酸水平上，4系PAstV与其他已报道的序列的同源性为61.2%-94.5%，而2系与同系同源序列的同源性为66.0%-71.6%。这是第一份关于PAstV和谱系4和谱系2在印度传播的报告。此外，系统发育分析表明PAstV菌株的多系起源，并建议在亚洲国家具有相似遗传配置的PAstV跨境流通。
Porcine astroviruses (PAstVs) have extended their distribution globally and have a high prevalence; however, their clinical significance is still under investigation. Thus far, information about their prevalence and diversity in the Indian pig population is unknown. This study is the first report on the prevalence and genetic characterization of PAstVs in diarrhoeic piglets in India. From January 2013 to December 2017, 757 samples were screened using an RT-PCR assay and PAstV infection was detected in 17.6% (133/757) pigs. Of the 133 positive samples, 79 (59.4%) were positive for PAstV alone, whereas 54 (40.6%) were found to be co-infected with porcine rotavirus A (PoRVA). Phylogenetic analysis of RdRp/capsid gene region revealed high genetic heterogeneity among PAstV sequences, with a predominance of PAstV lineage 4 and detection of lineage 2. The lineage 4 PAstVs exhibited 61.2%-94.5% sequence similarity at the nucleotide level to other reported sequences, whereas lineage 2 strain shared 66.0%-71.6% sequence identity with cognate sequences of the same lineage. This is the first report on PAstV and circulation of lineages 4 and 2 in India. Further, phylogenetic analysis indicates a multiphyletic origin of PAstV strains and suggests cross-border circulation of PAstVs with a similar genetic configuration in Asian countries.