Changes of phospho-growth-associated protein 43 (phospho-GAP43) in the zebrafish retina after optic nerve injury: A long-term observation
Changes of phospho-growth-associated protein 43 (phospho-GAP43) in the zebrafish retina after optic nerve injury: A long-term observation
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DOI：
10.1016/j.neures.2008.03.008
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发表时间：
2008-07-01
影响因子：
2.9
通讯作者：
Kato, Satoru
中科院分区：
文献类型：
--
作者：
Kaneda, Manabu;Nagashima, Mikiko;Kato, Satoru
金鱼是鱼类视神经再生的主要模式动物。与斑马鱼关系密切的斑马鱼是脊椎动物中枢神经系统遗传和发育研究中最受欢迎的模式系统。一些具有挑战性的视神经再生工作已经在斑马鱼身上完成。然而，斑马鱼显然缺乏关于视神经再生的长期知识。因此，在本研究中，我们跟踪观察了斑马鱼视神经切断100天后行为和生长相关蛋白43磷酸化形式(磷酸化形式GAP43)在斑马鱼视网膜中表达的变化。视神经运动反应在视神经切断后20~25天恢复较快，而追逐行为(学校教育行为)在视神经切断后80~100天恢复较慢。磷酸化GAP43的表达呈双相增加，1~2周为短高峰(12倍)，1~2个月为长平台(4倍)。视运动反应的恢复与生长轴突向顶盖的投射密切相关，而追逐行为的恢复与视网膜顶盖地形图的突触细化密切相关。目前的数据有力地表明，磷酸化的GAP43在鱼类视神经再生的早期和晚期都起着积极的作用。(C)2008年爱思唯尔爱尔兰有限公司和日本神经科学学会。保留所有权利：
The major model animal of optic nerve regeneration in fish is goldfish. A closely related zebrafish is the most popular model system for genetic and developmental studies of vertebrate central nervous system. A few challenging works of optic nerve regeneration have been done with zebrafish. However, knowledge concerning the long term of optic nerve regeneration apparently lacks in zebrafish. In the present study, therefore, we followed changes of zebrafish behavior and phosphorylated form of growth-associated protein 43 (phospho-GAP43) expression in the zebrafish retina over 100 days after optic nerve transection. Optomotor response was fast recovered by 20-25 days after axotomy whereas chasing behavior (a schooling behavior) was slowly recovered by 80-100 days after axotomy. The temporal pattern of phospho-GAP43 expression showed a biphasic increase, a short-peak (12 folds) at 1-2 weeks and a long-plateau (4 folds) at 1-2 months after axotomy. The recovery of optomotor response well correlated with projection of growing axons to the tectum, whereas the recovery of chasing behavior well correlated with synaptic refinement of retinotectal topography. The present data strongly suggest that phospho-GAP43 plays an active role in both the early and late stages of optic nerve regeneration in fish. (c) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved: