Steric course of the N-methylation in the biosynthesis of ergot alkaloids by Claviceps purpurea.
Steric course of the N-methylation in the biosynthesis of ergot alkaloids by Claviceps purpurea.
复制标题
麦角菌生物合成麦角生物碱中 N-甲基化的空间过程。
DOI：
10.1002/jobm.3620310209
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
Floss,HG
中科院分区：
文献类型：
--
作者：
Gröger,D;Gröger,L;D'Amico,D;He,MX;Floss,HG
使用手性甲基方法学，发现在由酶DMMet：二甲基烯丙基色氨酸N-甲基转移酶催化的麦角生物碱生物合成中的甲基化步骤以甲基构型的净反转进行。因此，该酶与所研究的大多数甲基转移酶一致，这些甲基转移酶在三元酶底物复合物中介导甲基从β Met 2）直接SN 2转移到受体亲核体。
Using the chiral methyl group methodology, the methylation step in the biosynthesis of ergot alkaloids catalyzed by the enzyme AdoMet:dimethylallyltryptophan N‐methyltransferase was found to proceed with net inversion of methyl group configuration. The enzyme thus conforms to the majority of methyltransferases studied which mediate a direct SN2 transfer of the methyl group from AdoMet2) to the acceptor nucleophile in a ternary enzyme substrate complex.