Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer
Enhanced transfection of polyplexes based on pluronic-polypropylenimine dendrimer for gene transfer
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基于 Pluronic-聚丙烯亚胺树枝状聚合物的多聚复合物的增强转染用于基因转移
DOI：
10.1007/s12272-009-1710-3
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发表时间：
2009-07-01
影响因子：
6.7
通讯作者：
Fang, Xiaoling
中科院分区：
文献类型：
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作者：
Hao, Junguo;Sha, Xianyi;Fang, Xiaoling
采用Pluronic P123对第三代阳离子树枝状聚合物聚丙烯亚胺（PPI）进行修饰，并研究其用于基因传递的性能。MTT法检测P123-PPI的细胞毒性，结果显示其细胞毒性远低于PPI。P123-PPI和PPI在N/P比为20：1时均能将质粒DNA凝聚成大小约为100 nm、zeta电位约为15 mV的纳米颗粒。纳米颗粒可以保护质粒DNA不被浓度为0.4U/μ g DNA的DNase I消化。纳米颗粒对50%胎牛血清和75 μ g/mL肝素钠诱导的解离具有抗性。使用P123-PPI/DNA纳米颗粒的SPC-A1细胞的转染效率远高于使用PPI/DNA纳米颗粒的转染效率。在制备P123-PPI/DNA纳米粒的过程中加入游离P123可显著提高10%胎牛血清存在下的转染效率。因此，P123-PPI/DNA复合物纳米粒可能是一种安全、高效、有前途的阳离子型基因载体。
Third generation cationic dendritic polymeric polypropyleneimine (PPI) was modified by Pluronic P123 and investigated for gene delivery. The cytotoxicity of P123-PPI was evaluated by the MTT assay and shown to be much lower than that of PPI alone. P123-PPI and PPI can both condense plasmid DNA into nanoparticles with a size of approximately 100 nm and a zeta potential of about 15 mV at the N/P ratio 20:1. The nanoparticles can protect plasmid DNA from being digested by DNase I at a concentration of 0.4 U/mu g DNA. The nanoparticles were resistant to dissociation induced by 50% fetal bovine serum and 75 mu g/mL sodium heparin. The transfection efficiency of SPC-A1 cells using P123-PPI/DNA nanoparticles was much higher than the transfection utilizing PPI/DNA nanoparticles. The addition of free P123 during the preparation of P123-PPI/DNA nanoparticles could significantly enhance the transfection efficiency in the presence of 10% fetal bovine serum. Therefore, P123-PPI/DNA complex nanoparticles may be a safe, efficient and promising cationic conjugate for gene delivery.