High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community.

High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community.
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在活性污泥衍生的微生物群落中携带质粒 pJP4 的转接合子的高度系统发育多样性。

DOI:
10.1016/j.femsle.2004.04.038
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发表时间:
2004
影响因子:
2.1
通讯作者:
M. Hausner
M. Hausner
中科院分区:
生物学4区
文献类型:
--
作者:
S. Bathe;M. Lebuhn;J. Ellwart;S. Wuertz;M. Hausner

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从恶臭假单胞菌(Pseudomonasputidadonor)与活性污泥来源的微生物群落的琼脂平板交配中分离出质粒pJP4的接合子,并通过部分16SrDNA测序进行鉴定。接合转移菌株属于变形菌门α-、β-、γ β-和γ-纲的多种属,主要是根瘤菌科、丛枝菌科和寡养单胞菌属。只有P。putida和Delftiaspp.菌株能够在2,4-D作为唯一碳源上生长。
Transconjugants of plasmid pJP4, originating from an agar plate mating of aPseudomonas putidadonor with an activated sludge-derived microbial community, were isolated and identified by partial 16S rDNA sequencing. The transconjugant strains belonged to a variety of genera of the α-, β-, γβ- and γ-classes of theProteobacteria, mostly to the familiesRhizobiaceaeandComamonadaceaeand the genusStenotrophomonas. OnlyP. putidaandDelftiaspp. strains were able to grow on 2,4-D as the sole carbon source.
DOI: 10.1073/pnas.97.22.11984
发表时间: 2000-10-24
影响因子: 11.1
作者:
Baird, GS;Zacharias, DA;Tsien, RY
通讯作者: Tsien, RY