Sertoli cells synthesize and secrete transferrin-like protein.
Sertoli cells synthesize and secrete transferrin-like protein.
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DOI：
10.1016/s0021-9258(18)43419-9
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发表时间：
1980-10
期刊：
影响因子：
--
通讯作者：
M. Skinner;M. Griswold
中科院分区：
文献类型：
--
作者：
M. Skinner;M. Griswold
支持细胞分泌的主要蛋白质之一（带 3）在十二烷基硫酸钠-聚丙烯酰胺凝胶上的分子量与大鼠血清转铁蛋白相对应。支持细胞分泌蛋白的制剂含有铁结合蛋白，其在天然聚丙烯酰胺凝胶上的电泳特性与血清转铁蛋白相似。针对支持细胞分泌蛋白的抗体含有能够免疫沉淀血清转铁蛋白的成分。血清转铁蛋白抗体与 [35S]-蛋氨酸标记的支持细胞分泌蛋白的主要成分（带 3）发生交叉反应并进行免疫沉淀。我们认为睾丸转铁蛋白是培养物中支持细胞的主要分泌产物。除了转铁蛋白之外，大鼠血清蛋白的抗血清还会免疫沉淀支持细胞分泌蛋白制剂中的另一种多肽。
One of the major proteins secreted by Sertoli cells (Band 3) has a molecular weight on sodium dodecyl sulfate-polyacrylamide gels which corresponds to rat serum transferrin. The preparation of Sertoli cell-secreted proteins contains an iron-binding protein with electrophoretic properties on native polyacrylamide gels similar to serum transferrin. Antibodies against Sertoli cell-secreted proteins contain a component which will immunoprecipitate serum transferrin. Antibodies to serum transferrin cross-react with and immunoprecipitate a major component (Band 3) of [35S]-methionine-labeled Sertoli cell-secreted proteins. We propose that testicular transferrin is a major secretory product of Sertoli cells in culture. In addition to transferrin, antiserum to rat serum proteins will immunoprecipitate one other polypeptide from preparations of Sertoli cell-secreted proteins.