The arcuate nucleus as a circumventricular organ in the mouse
The arcuate nucleus as a circumventricular organ in the mouse
复制标题
DOI：
10.1016/j.neulet.2010.10.019
复制
发表时间：
2011-01-07
影响因子：
2.5
通讯作者：
Ciofi, Philippe
中科院分区：
文献类型：
--
作者：
Ciofi, Philippe
本研究旨在探讨小鼠腹内侧弓状核对血液蛋白质渗透性的形态学相关性。首先，我们确定，高渗透性微血管检测在腹内侧弓状核使用大鼠单克隆抗体小鼠特异性内皮细胞表型（克隆MECA 32）最近被认为是一个标志物的内皮窗隔和以前显示标记室周器官。其次，在MECA 32强制使用的组织固定的温和条件下，我们观察到在用盐水快速冲洗血管后，内源性免疫球蛋白尤其保留在室周器官和腹内侧弓状核中。因此，腹内侧弓状核与经典的室周器官具有共同的特征。(C)2010爱思唯尔爱尔兰有限公司版权所有。
The present study searched for morphological correlates of the permeability of the ventromedial arcuate nucleus of the mouse to blood-borne proteins. First, we determined that highly permeable microvessels are detected in the ventromedial arcuate nucleus using a rat monoclonal antibody to a mouse-specific endothelial phenotype (clone MECA32) recently recognized as a marker of endothelial fenestral diaphragms and previously shown to label circumventricular organs. Second, in the mild conditions of tissue fixation mandatory for use of MECA32, we observed that after a rapid vascular flush with saline, endogenous immunoglobulins are especially retained in circumventricular organs and ventromedial arcuate nucleus. The ventromedial arcuate nucleus thus shares features in common with classical circumventricular organs. (C) 2010 Elsevier Ireland Ltd. All rights reserved.