BOVINE CHROMOGRANIN-A SEQUENCE AND DISTRIBUTION OF ITS MESSENGER-RNA IN ENDOCRINE TISSUES
BOVINE CHROMOGRANIN-A SEQUENCE AND DISTRIBUTION OF ITS MESSENGER-RNA IN ENDOCRINE TISSUES
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DOI：
10.1038/323082a0
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发表时间：
1986-09-04
期刊：
影响因子：
64.8
通讯作者：
GRIMES, M
中科院分区：
文献类型：
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作者：
IACANGELO, A;AFFOLTER, HU;GRIMES, M
嗜铬粒蛋白A包含在肾上腺髓质嗜铬细胞的储存囊泡中，并在内脏神经受到刺激时与儿茶酚胺一起释放1，2。嗜铬粒蛋白A类似于分泌蛋白I（SP-I），其是甲状旁腺的主要分泌蛋白3。嗜铬粒蛋白A/SP-I免疫反应性在从储存囊分泌肽激素的内分泌细胞中丰富4 -7。嗜铬粒蛋白可能通过结合囊泡内钙而在神经内分泌中起作用8，9。血清嗜铬粒蛋白水平在高血压10和内分泌肿瘤中升高8，10，11。我们在这里报告的分离和测序的cDNA编码牛嗜铬粒蛋白A，提供了第一个完整的一级结构的嗜铬粒蛋白。嗜铬粒蛋白A是一种强酸性蛋白质，在SDS-PAGE上的表观相对分子质量（Mr）为75，000，但实际Mr为48，000。肾上腺髓质、脑、垂体和甲状旁腺都是嗜铬粒蛋白A的合成部位。嗜铬粒蛋白A的一级结构和甲状旁腺中嗜铬粒蛋白mRNA的存在表明嗜铬粒蛋白A和SP-I是相同的。
Chromogranin A is contained in storage vesicles of chromaffin cells of the adrenal medulla and released with catecholamines when the splanchnic nerve is stimulated1,2. Chromogranin A is similar to secretory protein I (SP-I), a major secreted protein of the parathyroid3. Chromogranin A/SP-I immunoreactivity is abundant in endocrine cells that secrete peptide hormones from storage vesicles4–7. Chromogranins may act in neuroendocrine secretion by binding intravesicular calcium8,9. Serum levels of chromogranin are raised in hypertension10and endocrine neoplasia8,10,11. We report here the isolation and sequencing of a cDNA encoding bovine chromogranin A, providing the first complete primary structure of a chromogranin protein. Chromogranin A is a highly acidic protein with an apparent relative molecular mass (Mr) of 75,000 on SDS-PAGE, but an actualMrof 48,000. Adrenal medulla, brain, pituitary and parathyroid are all sites of synthesis of chromogranin A. The primary structure of chromogranin A, and the presence of chromogranin mRNA in the parathyroid, indicate that chromogranin A and SP-I are identical.