Methodology for coupling local application of dopamine and other chemicals with rapid in vivo electrochemical recordings in freely-moving rats
Methodology for coupling local application of dopamine and other chemicals with rapid in vivo electrochemical recordings in freely-moving rats
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DOI：
10.1016/s0165-0270(98)00158-7
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发表时间：
1999-02-01
影响因子：
3
通讯作者：
Zahniser, NR
中科院分区：
文献类型：
--
作者：
Gerhardt, GA;Ksir, C;Zahniser, NR
提出了构建和使用电极/微管组件的方法，该组件允许在体内电化学测量，并在未麻醉的自由运动大鼠中局部应用多巴胺（DA）和其他化学物质。在大鼠体内植入一根由碳纤维制成的伏安电极，电极密封在熔融硅胶管中，电极连接一对不锈钢导管，导管内插入熔融硅胶注射管，用于局部注射DA和其他化学物质。使用注射器驱动器结合流体旋转装置将溶液输送到注射管中，实现了纳升体积的精确输送。一种新设计的微型恒电位器通过模块化电话插孔组件连接到换向器，可以在自由移动的大鼠中进行高速计时电流计电化学记录。初步实验表征了伏安电极的体外电化学记录特性。体内研究还研究了自由运动大鼠纹状体和伏隔核中局部应用DA和钾诱发内源性DA的清除情况。此外，我们还研究了水合氯醛麻醉对伏隔核DA清除信号的影响。此外，在5天的时间内验证了该记录技术测量外源DA清除率的稳定性和重复性。最后，观察全身可卡因注射对伏隔核DA摄取和运动活动的影响。这些研究支持这样的结论，即本文所述的方法可以通过精确的微应用DA和其他化学物质结合动物行为的同步测量，在自由运动的大鼠中进行快速的计时安培电化学记录。(C) 1999 Elsevier Science B.V.版权所有
Methodology is presented for constructing and using an electrode/microcannulae assembly that allows in vivo electrochemical measurements coupled with local application of dopamine (DA) and other chemicals in the unanesthetized freely-moving rat. Rats were implanted with a voltammetric electrode constructed of a carbon fiber sealed in fused silica tubing attached to a pair of stainless steel guide cannulae, into which fused silica injection cannulae were inserted for local application of DA and other chemicals. Precise delivery of nanoliter volumes was accomplished using a syringe drive combined with a fluid swivel to deliver the solutions to the injection cannulae. A newly-designed miniature potentiostat connected to a commutator via a modular telephone jack assembly allowed for high-speed chronoamperometric electrochemical recordings in freely-moving rats. Initial experiments characterized the in vitro electrochemical recording characteristics of the voltammetric electrode. In vivo studies were also carried out to study clearance of locally-applied DA and of potassium-evoked endogenous DA in the striatum and nucleus accumbens of freely-moving rats. In addition, the effects of chloral hydrate anesthesia on DA clearance signals in the nucleus accumbens were investigated. Moreover, the stability and reproducibility of this recording technique for measuring exogenous DA clearance was verified over a period of 5 days. Finally, the concurrent effects of systemic cocaine injection on DA uptake in nucleus accumbens and locomotor activity were examined. These studies support the conclusion that the methodology described herein allows for rapid chronoamperometric electrochemical recordings in freely-moving rats with precise microapplications of DA and other chemicals combined with concurrent measures of animal behavior. (C) 1999 Elsevier Science B.V. All rights reserved.