Some Observations on the Use of Alum Precipitated Diphtheria Toxoid
Some Observations on the Use of Alum Precipitated Diphtheria Toxoid
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DOI：
10.2105/ajph.25.3.298
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发表时间：
1935-03-01
期刊：
影响因子：
--
通讯作者：
Harrison, W. T.
中科院分区：
文献类型：
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作者：
Harrison, W. T.
为了验证明矾沉淀类毒素的活性在于对相对不溶性沉淀物的吸收速度较慢的理论，作者解剖了豚鼠注射部位持续存在的结节，并将其重新注射到猪体内，其中抗毒素已被证明不存在。每周从2至4头猪身上取出结节，直至7日，将结节放入含盐溶液的砂浆中研磨，并注射到2头试验猪体内。试验猪6周后放血，每对猪抽取血清进行抗毒素检测。所有在第7周再次注射的人血清中都存在抗毒素，每毫升显示1/5单位。在注射沉淀类毒素后11天接种天花疫苗的人，在硬化结节周围观察到病毒的定位。在注射类毒素21天后接种疫苗的11名受试者中，9名显示正常的牛痘，2名显示病毒在类毒素注射部位有局部化的趋势。
To test the theory that the activity of alum precipitated toxoid lies in the slow rate of absorption of the comparatively insoluble precipitate, the author dissected the nodules which persist at the site of injection in guinea pigs, and reinjected them into pigs in which the absence of antitoxin had been demonstrated. The nodules, removed from 2 to 4 pigs each week up to the 7th, were ground in a mortar with salt solution and injected into 2 test pigs. The test pigs were bled after 6 weeks, the serum of each pair pooled and tested for antitoxin. Antitoxin was present in the serum of all, those reinjected at the 7th week showing 1/5 unit per cc. Localization of virus was observed around the indurated nodule in individ-uals vaccinated for smallpox 11 days after the injection of precipitated toxoid. 9 out of 11 subjects vaccinated 21 days after the injection of toxoid showed normal vaccinia and 2 indicated a tendency of the virus to localize at the site of the toxoid injection.