Use of an Eight-arm Radial Water Maze to Assess Working and Reference Memory Following Neonatal Brain Injury
Use of an Eight-arm Radial Water Maze to Assess Working and Reference Memory Following Neonatal Brain Injury
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DOI：
10.3791/50940
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发表时间：
2013-12-01
影响因子：
1.2
通讯作者：
Threlkeld, Steven W.
中科院分区：
文献类型：
--
作者：
Penley, Stephanie C.;Gaudet, Cynthia M.;Threlkeld, Steven W.
工作记忆和参考记忆通常使用陆基放射臂迷宫进行评估。然而，这种范式需要预先训练，食物匮乏，并可能引入气味线索混淆。八臂径向水迷宫旨在通过要求受试者使用迷宫外线索来定位逃生平台来同时评估参考和工作记忆性能，并弥补了陆基径向臂迷宫设计中观察到的限制。具体地说，受试者被要求避免在每个测试日使用以前用于逃生的手臂(工作记忆)，以及避免使用从未包含逃生平台的固定手臂(参考记忆)。在测试期间已经用于逃生的手臂(因此逃生平台已被移除)和参考记忆手臂的重新进入表明工作记忆缺陷。或者，参考记忆臂中的第一个条目表示参考记忆缺陷。我们使用这个迷宫来比较新生脑损伤大鼠和假对照组在诱导缺氧缺血后的表现，并在11天的测试后显示出工作记忆和参考记忆的显着缺陷。这个方案可以很容易地修改，以检查许多其他学习障碍模型。
Working and reference memory are commonly assessed using the land based radial arm maze. However, this paradigm requires pretraining, food deprivation, and may introduce scent cue confounds. The eight-arm radial water maze is designed to evaluate reference and working memory performance simultaneously by requiring subjects to use extra-maze cues to locate escape platforms and remedies the limitations observed in land based radial arm maze designs. Specifically, subjects are required to avoid the arms previously used for escape during each testing day (working memory) as well as avoid the fixed arms, which never contain escape platforms (reference memory). Re-entries into arms that have already been used for escape during a testing session (and thus the escape platform has been removed) and re-entries into reference memory arms are indicative of working memory deficits. Alternatively, first entries into reference memory arms are indicative of reference memory deficits. We used this maze to compare performance of rats with neonatal brain injury and sham controls following induction of hypoxiaischemia and show significant deficits in both working and reference memory after eleven days of testing. This protocol could be easily modified to examine many other models of learning impairment.