IN VITRO STUDIES ON POSSIBLE SYNERGISTIC ACTION BETWEEN PENICILLIN AND BACITRACIN.
IN VITRO STUDIES ON POSSIBLE SYNERGISTIC ACTION BETWEEN PENICILLIN AND BACITRACIN.
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青霉素和杆菌肽之间可能的协同作用的体外研究。
DOI：
10.1172/jci102169
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发表时间：
1949
期刊：
影响因子：
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通讯作者：
M. C. Bachman
中科院分区：
文献类型：
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作者：
M. C. Bachman
已经出现了许多出版物，描述了两种或多种抗菌物质的混合物的成功使用。在许多情况下，组合效应表明协同活性，而不仅仅是相加的。可能需要使用这些抗菌物质组合的原因有很多。对一种抗生素的耐药性很可能独立地遗传给第二种抗生素。因此，当每百万个细胞中有一个或两个细胞对抗生素A耐药时，它们对抗生素B也耐药的机会就非常低。当抗生素A和B一起使用时，每种抗生素的浓度都能抑制除耐药个体外的所有个体，除非在巨大的种群中，否则将没有幸存者。Klein和Kimmel-man发现，通过加入少量的磺胺嘧啶，可以大大减少体外链霉素耐药性变体的发展。此外，他们还发现，亚抑菌浓度的青霉素、链霉素和磺胺嘧啶联合使用时，在抑制细菌繁殖方面非常有效，并完全阻止了耐药变体的发展。他们的数据表明，抑制耐药变异体与青霉素、链霉素和磺胺嘧啶的协同作用之间存在直接相关性。
A number of publications have appeared de-scribing the successful use of mixtures oftwo or more antibacterial substances. In many instances the combined effect has indicated synergistic activity instead of being merely additive. There are a number of reasons why it may be desirable to use these combinations of antibacterial substances. Resistance to one antibiotic is prob-ably inherited independently to a second one. Thus when one or two cells per million are re-sistant to antibiotic A, the chancesthat they are also resistant to antibiotic B are very low. When antibiotics A and B are used together, eachin a concentration which will inhibit all but the resistant individuals, there will be no survivors, except in tremendous populations. Klein and Kimmel-man found that the development of streptomycin-resistant variants in vitro could be greatly reduced by the addition of small amounts of sulphadiazine. Furthermore, they found that subinhibi-tory concentrations ofpenicillin, streptomycin, and sulphadiazine, when combined, were highly effective in inhibiting bacterial multiplication and completely prevented the development of resistant variants. Their data indicate direct correlation between the inhibition of resistant variants and synergistic action with penicillin, streptomycin and sulphadiazine.