Is photobleaching necessary for Raman imaging of bone tissue using a green laser?
Is photobleaching necessary for Raman imaging of bone tissue using a green laser?
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DOI：
10.1016/j.bbamem.2006.02.022
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发表时间：
2006-07-01
影响因子：
3.4
通讯作者：
Morris, Michael D.
中科院分区：
文献类型：
--
作者：
Golcuk, Kurtulus;Mandair, GuiJit S.;Morris, Michael D.
拉曼显微光谱广泛用于肌肉骨骼组织研究。但荧光背景掩盖了骨组织的矿物质和基质成分的显着拉曼带。 532 nm 激光照射已被有效地用于去除皮质骨拉曼光谱中的荧光背景。光化学漂白在 2 小时后可减少超过 80% 的荧光背景，并且在 40 分钟内被发现是无损的。使用电子倍增偶电荷探测器（EMCCD）可以在1-5秒范围内获取骨组织的拉曼光谱，并在不到10分钟内获得拉曼图像。 (c) 2006 Elsevier B.V. 保留所有权利。
Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of cortical bone. Photochemical bleaching reduces over 80% of the fluorescence background after 2 h and is found to be nondestructive within 40 min. The use of electron multiplying couple charge detector (EMCCD) enables to acquire Raman spectra of bone tissues within 1-5 s range and to obtain Raman images less than in 10 min. (c) 2006 Elsevier B.V. All rights reserved.