Comparison of bleomycin and radiation in the G2 assay of chromatid breaks
Comparison of bleomycin and radiation in the G2 assay of chromatid breaks
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DOI：
10.1080/09553000310001596968
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发表时间：
2003-08-01
影响因子：
2.6
通讯作者：
Bryant, PE
中科院分区：
文献类型：
--
作者：
Adema, AD;Cloos, J;Bryant, PE
目的：比较博莱霉素和放射治疗在G2期染色单体断裂试验中的作用。关于G2染色单体断裂敏感性与癌症易感性的关系是否与G2染色单体放射敏感性试验(所谓的“G2试验”)相同，文献中存在争议。虽然博莱霉素是一种仿射线制剂，但它与放射损伤的诱导方式不同。材料和方法：来自两名头颈部鳞状细胞癌患者、两名乳腺癌患者、两名共济失调毛细血管扩张症患者和两名正常对照的EB病毒永生化淋巴母细胞系。用0.3-Gy射线或5mU·ml(-1)博莱霉素照射细胞，观察细胞染色体损伤情况。结果：两种不同的损伤诱导剂之间存在很强的正相关关系(r=0.99；p<；0.001)。这种相关性对于每个细胞的断裂和每个细胞的总像差都是相似的。在染色单体断裂评分中加入间隙与数据的较高变异性相关，但这并不影响本研究的结果。结论：博莱霉素和辐射产生的敏感性表型与G2染色单体断裂试验所确定的相同。因此，当不存在辐射设备时，博莱霉素似乎是这种类型分析的辐射的良好替代品。
Purpose: To compare bleomycin with radiation in the G2 chromatid break assay. Controversy exists in the literature about whether G2 bleomycin chromatid-break sensitivity links with cancer predisposition in the same way as the G2 chromatid radiosensitivity test (the so-called 'G2 assay'). Although bleomycin is referred to as a 'radiomimetic' agent, it differs from radiation in the way the damage is induced.Materials and methods: Epstein-Barr virus-immortalized lymphoblastoid cell lines from two head and neck squamous cell carcinoma patients, two breast cancer patients, two ataxia-telangiectasia patients and two normal control persons were used. Chromosomal damage was determined in cells exposed to 0.3-Gy radiation or 5 mU ml(-1) bleomycin. The numbers of chromatid breaks per cell and of aberrations per cell (i.e. breaks and gaps) were determined.Results: A strong positive correlation was found between the two different damage inducers (r = 0.99; p < 0.001). This correlation was similar for both the breaks per cell and the total aberrations per cell. Inclusion of gaps in the scoring of chromatid breaks was associated with a higher variability of the data, but this did not influence the outcome of this study.Conclusions: Both bleomycin and radiation give the same sensitivity phenotypes as determined by the G2 assay of chromatid breaks. Thus, when no radiation facility is present, bleomycin seems to be a good alternative to radiation for this type of assay.