THE PROLONGATION OF MITOTIC STAGES IN SV40-TRANSFORMED VS NONTRANSFORMED HUMAN FIBROBLAST CELLS
THE PROLONGATION OF MITOTIC STAGES IN SV40-TRANSFORMED VS NONTRANSFORMED HUMAN FIBROBLAST CELLS
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DOI：
10.1002/jcp.1041130206
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发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
GRASCH, SD
中科院分区：
文献类型：
--
作者：
SISKEN, JE;BONNER, SV;GRASCH, SD
早期对许多正常细胞和肿瘤细胞有丝分裂阶段持续时间和中期与前期比例的研究表明，有丝分裂过程在肿瘤发生过程中发生改变。这些变化的性质及其对每个有丝分裂阶段的影响尚不清楚。利用时间推移电影显微摄影技术，将SV40/WI-38和SV40/WI-26细胞与正常细胞和其他未转化的人成纤维细胞的有丝分裂期进行比较。在WI-38和SV40/WI-38细胞的固定制剂中检测单个有丝分裂阶段的相对频率。转化细胞的中期持续时间增加，与Wi-38和其他未转化细胞相比，SV40/Wi-38细胞的中期持续时间增加了3.0- 4.7倍。其他阶段也会延长，但程度较轻。在许多肿瘤中观察到的中期/前期比值的增加可能是由于中期持续时间的增加，而不是由于较短的前期，而在恶性肿瘤中常见的有丝分裂指数的增加，有时被用作生长速度的指标，至少部分是由于有丝分裂阶段的延长。
Early studies on the duration of mitotic stages and on metaphase-to-prophase ratios in a number of normal and neoplastic cells indicated that the process of mitosis becomes altered during the course of oncogenesis. The nature of these changes and their effects on each of the mitotic stages are still unclear. With the use of time lapse cinemicrography, the durations of mitotic stages of SV40/WI-38 and SV40/WI-26 cells were compared to those of their normal counterparts and to other nontransformed human fibroblasts. The relative frequencies of the individual mitotic stages were examined in fixed preparations of WI-38 and SV40/WI-38 cells. Metaphase durations are increased in the transformed cells and as much as 3.0- to 4.7-fold in SV40/Wi-38 cells compared to WI-38 and other nontransformed cells. Other stages are also prolonged, to lesser degrees. Increased metaphase/prophase ratios observed in many tumors may be due to increases in duration of metaphase rather than to shorter prophases, and increased mitotic indices commonly observed in malignant tumors and sometimes used as an index of growth rate are at least in part due to the prolongation of mitotic stages.